Comparative Study of the Haemagglutination Capabilities of Lectin Extracted from Submerged Cultures of Wild and Mutant Strains of Schizophyllum commune
Haemagglutination
studies on lectin from both wild (SCW) and mutant strains (SCM1, SCM2, SCM3) of Schizophyllum commune using human
blood were explored in this study after six days of submerged fermentation.
Haemagglutination assay showed that the lectin from all the strains showed
slight discrimination in their haemmaglutinating activity against human blood
group with strong exhibition of agglutination with blood group O while SCM3 and
SCW showed the highest and lowest haemaglutinating activity respectively with a
titre score from 4 - 256 HA. Absolute loss of haemagglutination activity was
shown by all the four S. commune strains tested following exposure to
CuSO4 and NH4SO4 at 800 mM but optimized by
KCl, MgCl2 at 100 mM. Optimal pH for maximal haemagglutination
activity was observed at 7.0 for SCW, 6.0 for SCM1 and 8.0 for SCM2 while SCM3
distinctly showed 5.5 and 8.5. Except for SCM3 the thermo stability of
haemagglutinating activity was found to improve with the duration of UV
irradiation. Inhibitory study in the presence of sugar showed that the
partially purified protein from all the strains of S. commune in this study was mannose dependent lectin.
Cite this paper
Awoyinka, O. A. , Aina, D. A. , Oloke, J. K. , Majolagbe, O. N. and Akoni, O. I. (2014). Comparative Study of the Haemagglutination Capabilities of Lectin Extracted from Submerged Cultures of Wild and Mutant Strains of Schizophyllum commune. Open Access Library Journal, 1, e993. doi: http://dx.doi.org/10.4236/oalib.1100993.
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