Reference Genes for Real-time Fluorescence Quantitative PCR in Camellia sinensis
茶树实时荧光定量PCR分析中内参基因的选择

The selection of a suitable reference gene is an important prerequisite for successful gene expression analysis by real-time fluorescence quantitative PCR (qPCR). We investigated the expression stability of 4 endogenous candidate genes (18S rRNA, GAPDH, β-actin and α-tubulin) in qPCR experiments in different organs and tissues, including buds, leaves, young roots, stem, petals, seeds, and callus, of the tea plant Camellia sinensis (L.) O. Kuntze. The analysis with GeNorm and NormFinder algorithms revealed that β-actin could be used as a reference gene for organs and tissues and GAPDH for mature leaves and callus.