A real-time PCR method for rapid detection of Gymnodinium sanguineum
红色裸甲藻实时定量PCR快速检测方法的建立(英文)

For detecting the harmful algal bloom(HAB)species sensitively and rapidly,Gymnodinium sanguineum was taken as the object,and the rapid detection method-RFQ-PCR(real-time fluorescent quantitative polymerase chain reaction)technique was applied to the HAB species detection.Firstly,gene specific primers and a TaqMan DNA probe were designed based on the sequence of 18S rDNA cloned from G.sanguineum.Then,using the primers and probe combination.a RFQ-PCR method was developed for quantitative detection of G.sanguineum.a harmful algae bloom species.The RFQ-PCR assay data showed good agreement with traditional microscope counting methods,suggesting that RFQPCR may be a useful method for detecting G.sanguineum.The detection threshold tested was much lower than that of G.sanguineum HAB.so this technique will be useful for forecasting and preventing G.sanguineum HAB.