ANTIMICROBIAL ACTIVITY OF 4{[(7-CHLORO-2-NITRO PHENOTHIAZINE-10-YL)-PHENYL-METHYL]-AMINO}-BENZOIC ACID DERIVATIVES

The antimicrobial activity of novel synthesized compounds was evaluated by Disc- Diffusion method. Compounds IVc, IVf and IV-i showed better, while IVb, IVd and IVh showed moderate and rest showed poor antimicrobial activity. The Disc Diffusion method of assay of drug potency is based on the measurement of the zone of microbial growth inhibition surroundings discs containing various concentrations of test compounds, which are placed on the surface of a solid nutrient previously inoculated with the culture of suitable microorganism. Inhibition produced by the test drug is compared with that produced by known concentration of reference standard. The in vitro screening was carried out using selected microbes like B. subtilis (NCIM) 2063, Gram +ve), S. aureus(NCIM 2079, Gram +ve), E.coli (NCIM 2931, Gram -ve).