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棉花学报  2013 

增强UV-B辐射对棉花幼苗氮代谢生理特性的影响

DOI: 1002-7807(2013)06-0525-08, PP. 525-532

Keywords: UV-B辐射,棉花,幼苗,氮代谢

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Abstract:

在室内水培条件下,人工模拟紫外辐射(Ultraviolet-B),研究UV-B辐射对两个转Bt基因抗虫棉材料29317系和29312系幼苗物质积累和氮代谢生理特性的影响。实验设置3个处理,UV-B辐射剂量分别是0kJ·m-2·d-1、2.7kJ·m-2·d-1、5.4kJ·m-2·d-1。结果表明,增强UV-B辐射可以降低棉花幼苗地上部生物量的积累而对地下部没有显著影响;低强度的UV-B辐射(2.7kJ·m-2·d-1)对全氮的含量没有影响,而高强度的UV-B辐射(5.4kJ·m-2·d-1)可以显著降低全氮的含量;UV-B辐射可以增加棉花幼苗叶片中硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、蛋白酶、肽酶的活性,提高可溶性蛋白和游离氨基酸的含量。UV-B辐射提高棉花幼苗叶片中蛋白质的合成和降解速度,使氮代谢加强。棉花幼苗通过加速氮循环途径来适应UV-B辐射,降低伤害程度。

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