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甘蔗宿根矮化病菌巢式PCR检测

, PP. 508-512

Keywords: 甘蔗,宿根矮化病菌,巢式PCR

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Abstract:

为了探索甘蔗宿根矮化病快速检测技术,以细菌16S-23SrDNA内转录间隔区(ITS)通用引物ITS1/ITS2为第1轮引物,以甘蔗宿根矮化病菌特异引物Lxx1/Lxx2为第2轮引物,建立甘蔗宿根矮化病菌巢式PCR检测技术。巢式PCR能扩增出438bp的目的条带,其核苷酸序列与巴西、澳大利亚及美国甘蔗宿根矮化病菌分离物核苷酸序列同源率为100%或99.8%;其检测灵敏度为10fg甘蔗宿根矮化病菌基因组DNA,较常规PCR提高100倍;样品检测结果也表明巢式PCR检测灵敏度明显优于常规PCR,可用于+1片嫩叶和心叶等微量病菌样品的检测。

References

[1]  马丁,阿伯特,休兹C G. 世界甘蔗病害(第一卷). 陈庆龙译. 北京:农业出版社,1982
[2]  Rago A M, Acreche M M, Sopena R A, et al. A survey of ratoon stunting disease (Leifsonia xyli subsp.xyli) in commercial sugarcane fields at Tucuman (Argentina). Sugar Cane International, 2004, 22(6): 12-14
[3]  Davis M J, Gillaspie A G, Jr Harris R W, et al. Ratoon stunting disease of sugarcane: isolation of the causal bacterium. Science, 1980, 210: 1365-1367
[4]  沈万宽,郑学文,陈仲华,等. 湛江农垦蔗区甘蔗宿根矮化病调查研究. 中国农学通报,2007,23(4):387-391
[5]  Fegan M, Croft B J, Teakle D S, et al. Sensitive and specific detection of Clavibacter xyli subsp.xyli, causal agent of ratoon stunting disease of sugarcane, with a polymerase chain reaction-based assay. Plant Pathology, 1998, 47(4): 495-504
[6]  Pan Y B, Grisham M P, Burner D M, et al. A polymerase chain reaction protocol for the detection of Clavibacter xyli subsp.xyli, the causal bacterium of sugarcane ratoon stunting disease. Plant Disease, 1998, 82(3): 285-290
[7]  Taylor P W J, Petrasovite L A, Velde R, et al. Development of PCR-based markers for detection of Leifsonia xyli subsp.xyli in fibrovascular fluid of infected sugarcane plants. Australasion Plant Pathology, 2003, 32(3): 367-375
[8]  沈万宽,周国辉,邓海华,等. 甘蔗宿根矮化病菌PCR检测及目的片段核苷酸序列分析. 中国农学通报,2006,22(12):413-416
[9]  刘睿,沈万宽,陈仲华,等. 甘蔗健康苗圃宿根矮化病检测及结果分析. 中国农学通报,2011,27(19):239-243
[10]  李河,宋光桃,何末军,等. 巢式PCR检测油茶根腐病菌的研究. 浙江林学院学报, 2009, 26(6):849-853
[11]  陈庆河,李本金,兰成忠,等. 双重PCR检测马铃薯晚疫病菌和青枯病菌方法的建立及应用. 植物病理学报,2009,39(6):578-583
[12]  Liu J N, He Li, Zhou G Y. Specific and rapid detection of Camellia oleifera anthracnose pathogen by nested-PCR. African Journal of Biotechnology, 2009, 8(6): 1056-1061
[13]  毛钧,蔡青,李文凤,等. 甘蔗宿根矮化病巢式PCR检测体系. 中国糖料,2009(2):31-32
[14]  Woese C R, Gutell R, Gupta R, et al. Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids. Microbiology Review, 1983, 47(4): 621-669
[15]  Brosius J, Dull T J, Noller H F. Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America, 1980, 77(1):201-204
[16]  彭学贤. 植物分子生物技术应用手册. 北京:化学工业出版社,2006
[17]  Peres N A R, Kuramae E E, Dias M S C, et al. Identification and characterization of Colletotrichum spp. affecting fruit after harvest in Brazil. Journal of Phytopathology, 2002, 150(3): 128-134
[18]  唐建辉,王伟,王源超.西瓜炭疽病菌Colletotrichum orbiculare的分子检测.中国农业科学,2006,39(10):2028-2035
[19]  沈万宽,邓海华,周国辉,等. 应用DB-EIA技术快速检测甘蔗宿根矮化病研究. 植物保护,2007,33(2):110-113
[20]  沈万宽,陈仲华,杨湛端,等. 热水处理防治甘蔗宿根矮化病的效果及对再生植株影响研究. 云南农业大学学报(自然科学版),2008,23(4):474-478

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