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水稻矮缩病毒基因组及毒粒三维结构的研究进展

, PP. 207-213

Keywords: 水稻矮缩病毒,基因组分析,三维结构,复制与组装

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Abstract:

水稻矮缩病毒(Ricedwarfvirus,RDV)为双层壳、二十面体的双链RNA病毒,是水稻的重要病原物之一。文中比较了RDV中国福建分离物与日本分离物基因组各片段核苷酸序列的相似性,发现RDV两个不同分离物基因组相应片段的相似性均在92%以上,最高可达96%;分析了相应基因组片段编码的结构蛋白P1、P2、P3、P5、P7、P8及以前一直被认为是非结构蛋白的P9和非结构蛋白Pns4、Pns6、Pns10、Pns11和Pns12在RDV复制、组装过程中的功能;回顾了RDV粒子三维结构的研究概况,对RDV粒子内、外层衣壳的晶体学结构作了较详细的描述;简要介绍了RDV复制与组装的机制,发现核心蛋白与dsRNA间相互作用成为一个单元是病毒RNA的分拣及包装到病毒粒子核心内的可能机制。同时,指出基于PDR的植物抗病毒基因工程在水稻矮缩病毒防治上的可能性。

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