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复合式膜生物反应器中胞外聚合物提取方法综合评价

Keywords: 膜生物反应器,胞外聚合物,提取方法,三维荧光光谱,凝胶渗透色谱

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Abstract:

采用高速离心法、超声法、阳离子交换树脂法(CER)、加热法4种方法提取复合式膜生物反应器(HMBR)中污泥胞外聚合物(EPS),利用化学分析方法结合三维荧光光谱法(EEM)和凝胶渗透色谱法(GPC)对4种方法进行评价。结果表明,采用60℃,30min加热提取效果最好,而且对细胞破坏小。通过三维荧光光谱图表明4种方法对于类富里酸均有较好的提取效果;对于类腐植酸和类蛋白质,热提取法优于其他3种方法。凝胶渗透色谱法分析表明4种方法提取的EPS分子量(MW)分布变化差别不大,分子量分布范围为100~1000000,主要集中在100~10000之间。

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