全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

马铃薯类黄酮-3-O-葡萄糖基化酶基因的克隆与表达分析

DOI: 10.7668/hbnxb.2009.04.003, PP. 11-16

Keywords: 花色苷,cDNA克隆,表达模式,Solanumtuberosum,类黄酮-3-O-葡萄糖基化酶

Full-Text   Cite this paper   Add to My Lib

Abstract:

根据茄科植物花色苷生物合成相关基因的资料,设计简并引物,通过RT-PCR的方法从马铃薯(Solanumtuberosumcv.Chieftain)紫色芽的cDNA中克隆到类黄酮-3-O-葡萄糖基化酶基因(3GT)的全长cDNA.序列分析表明,这个3GT基因编码的多肽为448个氨基酸残基,在氨基酸水平上与茄科的矮牵牛和茄子的一致性最高,均为7%,多重比较和系统发育分析均表明,该基因为3GT家族中的一个新成员.用半定量RT-PCR进行了表达分析表明,3GT在根、叶和块茎中的表达量远高于茎和花;此外,3GT在Chieftain的红色愈伤组织中表达而在非红色愈伤组织中不表达,可见,3GT的表达与花色苷的积累是相关的.

References

[1]  Schwinn K L,Davies K M,Deroles S C,et al.Expression of an Antirrhinum majus UDP-glucose:flavonoid-3-O-glucostransferase transgene alters flavonoid glycosylation and acylation in lisianthus (Eustoma grandiflorum Grise.)[J].Plant Sci99725:53-61.
[2]  Yamazaki M,Yamagishi E,Gong Z,et al.Two flavoniod glucosyltransferases from Petunia Hybrida:molecular cloning,biochemical properties and developmentally regulated expression[J].Plant Mol Biol,2002,48:401-411.
[3]  孙明霞,王宝增,范海,等.叶片的花色素苷及其对植物适应环境的意义[J].植物生理学通讯,2003,39 (6):688-694.
[4]  张树军,李元,韩冰.矮牵牛的组织培养与快繁[J].内蒙古农业科技,2001(4):5-6.
[5]  高银.植物抗逆机制与基因工程研究进展[J].内蒙古农业科技,2007(5):75-78.
[6]  杨晓清,李正英,杨志华.荞麦皮黑色素提取条件及基本特性的研究[J].内蒙古农业科技,2000(6):17-18.
[7]  徐亚民,马越,赵晓燕,等.紫苏等4种天然色素抗氧化能力的比较[J].华北农学报,2007,22(2):187-190.
[8]  Kobayashi S,Ishimaru M,Ding C K,et al.Comparison of UDP-glucose:Flavonoid 3-O-glucosyltransferase (UFGT)gene sequences between white grapes (Vitis Vinifera)and their sports with red skin[J].Plant Sci,200160:543-550.
[9]  Afifi M,El-kdreamy A,Legrand V,et al.Control of anthocyanin biosynthesis pathway gene expression by eutypine,a toxin from Eutypa lata,in grape cell tiussue cultures[J].J Plant Physiol,200360:971-975.
[10]  Nakatsuka T,Nishihara M,Mishiba K,et al.Two different muations are involved in the formation of white-flowered gentian plants[J].Plant Sci,200569:949-958.
[11]  Holton T,Cornish E C.Genetics and biochemistry of anthocyanin biosynthesis[J].Plant Cell995,7:1071-1083.
[12]  卢其能,杨清,却志群,等.马铃薯CHS基因cDNA的克隆与表达分析[J].华北农学报,2008,23(5):17-22.
[13]  Lu Q N,Yang Q.cDNA cloning and expression of anthocyanin biosynthetic genes in wild potato (Solanum pinnatisectum)[J].African Journal of Biotechnology,2006,5(10):811-818.
[14]  De Jong W S,De Jong D M,De Jong H.An allele of dihydroflavonol 4-reductase associated with the ability to produce red anthocyanin pigments in potato (Solanum tuberosum L.)[J].Theor Appl Genet,200307:1375-1383.
[15]  Jung C S,Griffiths H M,De Jong D M,et al.The potato plocus codes for flavonoid 3'',5''-hydroxylase[J].Theor Appl Genet,200510:269-275.
[16]  Knud L.Molecular cloning and characterization of cDNAs encoding cinnamoyl CoA reductase (CCR)from barley (Hordeum vulgare)and potato (Solanum tuberum)[J].J Plant Physiol,200461:105-112.
[17]  Mancinelli A L,Rossi F,Moroni A.Cryptochrome,phytochrome,and anthocyanin production[J].Plant physiol991,96:1079-1085.
[18]  Yamazaki M,Gong Z,Fukuchi-Mizutani M,et al.Molecular cloning and biochemical characterization of a novel anthocyanin 5-O-glucosyltransferase by mRNA differential display for plant forms regarding anthocyanin[J].J Biol Chem999,274:7405-7411.
[19]  Dooner H K,Robins T P,Jorgensen R A.Genetic and developmental control of anthocyanin biosynthesis[J].Annu Rev Genet991,25:173-199.
[20]  Hung C Y,Murray J R,Ohmann S O,et al.Anghocyanin accumulation during potato tuber development[J].J Amer Soc Hort Sci99722 (1):20-23.
[21]  Honda C,Kotoda N,Wada M,et al.Anthocyanin biosynthetic genes are coordinately expressed during red coloration in apple skin[J].Plant Physiol Biochem,2002,40:955-962.
[22]  Tanaka Y,Katsumoto Y,Brugliera F,et al.Genetic engineering in floriculture[J].Plant Cell,Tissue and Organ Culture,2005,80:1-24.

Full-Text

Contact Us

[email protected]

QQ:3279437679

WhatsApp +8615387084133