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马铃薯类黄酮-3-O-葡萄糖基化酶基因的克隆与表达分析

DOI: 10.7668/hbnxb.2009.04.003, PP. 11-16

Keywords: 花色苷,cDNA克隆,表达模式,Solanumtuberosum,类黄酮-3-O-葡萄糖基化酶

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Abstract:

根据茄科植物花色苷生物合成相关基因的资料,设计简并引物,通过RT-PCR的方法从马铃薯(Solanumtuberosumcv.Chieftain)紫色芽的cDNA中克隆到类黄酮-3-O-葡萄糖基化酶基因(3GT)的全长cDNA.序列分析表明,这个3GT基因编码的多肽为448个氨基酸残基,在氨基酸水平上与茄科的矮牵牛和茄子的一致性最高,均为7%,多重比较和系统发育分析均表明,该基因为3GT家族中的一个新成员.用半定量RT-PCR进行了表达分析表明,3GT在根、叶和块茎中的表达量远高于茎和花;此外,3GT在Chieftain的红色愈伤组织中表达而在非红色愈伤组织中不表达,可见,3GT的表达与花色苷的积累是相关的.

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