|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
西安市典型景观水体水质与微生物种群结构多样性
DOI: 10.3724/SP.J.1145.2013.12003, PP. 697-703
Keywords: 景观水体,水体水质,微生物群落,巢式pcr-dgge,克隆测序
Abstract:
选取西安市区9个景观水体，在分析水体水质指标基础上，运用巢式pcr-dgge和克隆测序技术研究景观水体细菌和真菌的群落结构多样性.结果表明，9个景观水体均为劣ⅴ类水.水质指标中总氮（tn）含量最高为丰庆公园，为11.286mg/l，是曲江池的5倍；硝氮（no3-n）含量最高为革命公园，为5.614mg/l，是曲江池的5.6倍；氨氮（nh4-n）含量最高为丰庆公园，为3.442mg/l，是大明宫太液池的12倍；高锰酸盐指数（codmn）最高为丰庆公园，为38.886mg/l，是曲江池的3.2倍.巢式pcr-dgge图谱主成分分析（pca）结果显示，莲湖公园、永阳公园和大明宫太液池水体细菌种群相似度高，丰庆公园、新纪元公园、曲江池和革命公园水体细菌种群多样性相似，而兴庆湖水体细菌群落结构与其他水体差异显著（p<0.05）.莲湖公园、永阳公园和大明宫太液池水体细菌种群结构主要受水体no3-n、tn和do含量的调控，其中no3-n含量影响显著；nh4-n含量对革命公园、新纪元公园和曲江池水体细菌种群影响显著，其中no3-n和codmn呈现负相关关系.codmn含量对兴庆湖水体细菌种群多样性影响显著.大明宫太液池、莲湖公园、丰庆公园、新纪元公园和兴庆湖水体真菌种类高度相似，革命公园水体真菌群落结构与其他水体存在差异，种群受到tn、no3-n和nh4-n的综合调控.no2-n含量对永阳公园水体真菌种群影响最大，同时与酸碱度（ph）和溶解氧（do）含量呈现负相关.茎点霉属（phomasp.）为革命公园水体中优势真菌.可见，西安市典型景观水体悬浮细菌和真菌群落结构特征受到不同水质指标的综合调控.表4图2参27
| [1] | 1梁燕珍,孙国萍,岑英华,郭俊.生物技术在微污染景观水处理中的应用[j].环境科学与技术,2005,28(2):88-89[liangyz,sungp,cenyh,guoj.applicationofbiotechnologytotreatmentofmicro-pollutionlandscapewater[j].environscitechnol,2005,28(2):88-
|
| [2] | 2蔡昌凤,徐建平.景观水微污染控制[j].安徽工程科技学院学报,2003,18(1):1-3[caicf,xujp.landscapewaterpollutioncontrol[j].janhuiuniv(natscied),2003,18(1):1-
|
| [3] | 3niuy,shenh,chenj,xiep,yangx,taom,mazm,qim.phytoplanktoncommunitysuccessionshapingbacterioplanktoncommunitycompositioninlaketaihu,china[j].waterres,2011,45(14):4169-4182
|
| [4] | 11彭青,谢冰,袁琦,黄智婷,崔璐璐,王文婷.青草沙水库蓄水期间细菌群落结构变化的初步研究[j].环境科学,2012,33(10):3634-3640[pengq,xieb,yuanq,huangzt,cuill,wangwt.preliminarystudyonthechangesofbacterialcommunitystructureinqingcaoshareservoirduringwaterstorageperiod[j].envirolsci,2012,33(10):3634-
|
| [5] | 12wul,yuyh,zhangtl,fengws,zhangxa,liw.pcr-dggefingerprintinganalysisofplanktoncommunitiesanditsrelationshiptolaketrophicstatus[j].intrevhydrobiol,2009,94(5):528-541
|
| [6] | 13赵兴青,杨柳燕,陈灿,肖琳,蒋丽娟,马�,朱昊巍,于振洋,尹大强等.pcr-dgge技术用于湖泊沉积物中微生物群落结构多样性研究[j].生态学报,2006,26(11):3610-3616[zhaoxq,yangy,chenc,xiaol,jianglj,maz,zhuhw,yuzy,yindq.studyonthemicrobialdiversityinlakesedimentsbythemethodofpcr-dgge[j].actaecolsin,2006,26(11):3610-
|
| [7] | 14ufnarja,wangsy,christiansenjm,yampara-iquiseh,carsonca,ellenderrd.detectionofthenifhgeneofmethanobrevibactersmithii:apotentialtooltoidentifysewagepollutioninrecreationalwaters[j].japplmicrobiol,2006,101(1):44-52
|
| [8] | 15杜萍,刘晶晶,沈李东,胡宝兰,曾江宁,陈全震,寿鹿,廖一波.biolog和pcr-dgge技术解析椒江口沉积物微生物多样性[j].环境科学学报,2012,32(6):1436-1444[dup,liujj,shenld,hubl,zengjn,chenqz,shoul,liaoyb.diversityofmicroorganismsinsedimentsofthejiaojiangestuaryasestimatedbybiologandpcr-dgge[j].actascicircumst,2012,32(6):1436-
|
| [9] | 16jin,pengb,wangg,wangs,pengx.universalprimerpcrwithdggeforrapiddetectionofbacterialpathogens[j].jmicrobiolmethods,2004,57(3):409-413
|
| [10] | 17justn,kirychuks,gilberty,létourneauv,veillettem,singhb,duchainec.bacterialdiversitycharacterizationofbioaerosolsfromcage-housedandfloor-housedpoultryoperations[j].environres,2011,111(4):492-498
|
| [11] | 18uchidam,amakasuh,satohy,muratam.combinationsoflacticacidbacteriaandyeastsuitableforpreparationofmarinesilage[j].fishsci,2004,70(3):507-517
|
| [12] | 20wuzl,guowy,qiujz,huangtp,lixb,guanx.cloningandlocalizationofvip3agnelakesandglacialmeltwaters,mounteverest[j].femsmicribiollett,2006,265(1):98-105
|
| [13] | 21刘乐冕,杨军,余小青,余正,张永雨,田野,张冬红.厦门后溪流域沿城乡梯度浮游细菌多样性及其与环境因子的关系[j].应用与环境生物学报,2012,18(4):591-598[liulm,yangj,yuxq,yuz,zhangyy,tiany,zhangdh.bacterioplanktondiversityinxiamenhouxiriverwatershedalonganyrban-ruralgradientanditsrelationtoenvironmentalfactors[j].chinjapplenvironbiol,2012,18(4):591-
|
| [14] | 22张淑娜.营养盐对天津市景观水体环境质量的影响[j].天津师范大学学报:自然科学版,2007,27(2):39-42[zhangsn.influenceofnutrientsaltontheenvironmentalqualityofscenicwaterbodyintianjin[j].jtianjinnormuniv(natscied),2007,27(2):39-
|
| [15] | 23fengs,duanxm.changingofmicrobialcommunityinalargeeutrophicationshallowlake[j].procediaenvironsci,2011,10:1416-1424
|
| [16] | 24wilhelmsw,farnsleyse,lecleirgr.therelationshipsbetweennutrients,cyanobacterialtoxinsandthemicrobialcommunityintaihu(laketai),china[j].harmfulalgae,2011,10:207-215
|
| [17] | 25黄江丽,郑庆子.松花江流域吉林江段微污染水监测及水质变化规律[j].吉林化工学院学报,2009,26(3):25-27[huangjl,zengqz.changelawofwaterqualityandmonitoringformicro-pollutedwaterinjilinbasinsectionofsonghuariver[j].jjilininstchemtechnol(natscied),2009,26(3):25-
|
| [18] | 26屠腾,李蕾,毛冠男,王莹莹.利用细胞计数手段和dgge技术分析松花江干流部分地区的细菌种群多样性[j].生态学报,2012,32(11):3505-3515[tut,li,maogn,wangyy.analysisofbacterialdiversityinthesonghuariverbasedonnestedpcranddgge[j].actaecolsin,2012,32(11):3505-
|
| [19] | 27有小娟,李秋芬,张艳,徐勇,焦海峰.象山港内西沪港海域沉积环境细菌群落结构的时空变化及其主要环境影响因子[j].应用与环境生物学报,2013,19(5):742-749[youxj,liqf,zhangy,xuy,jiaohf.spatialandtemporalvariationofbacterialcommunitystructuresandtheirmainenvironmentalinfluencingfactorsinsedimentenvironmentofxihuareainxiangshanbay[j].chinjapplenvironbiol,2013,19(5):742-
|
| [20] | 4metheba,zehrjp.diversityofbacterialcommunitiesinadirondacklakes:dospeciesassemblagesreflectlakewaterchemistry[j].hydrobiology,1999,401:77-96
|
| [21] | 5jiangjg,shenyf.developmentofthemicrobialcommunitiesinlakedonghuinrelationtowaterquality[j].environmonitassess,2007,127:227-236
|
| [22] | 6learg,boothroydikg,turnersj,lewisgd.acomparisonofbacteriaandbenthicinvertebratesasindicatorsofecologicalhealthinstreams[j].freshwaterbiol,2009,54(7):1532-1543
|
| [23] | 7parrlhw,dyblej,moisanderph,noblert,piehlermf,pinckneyjl,steppetf,twomeyluke,valdeslm.microbialindicatorsofaquaticecosystemchange:currentapplicationstoeutrophicationstudies[j].femsmicrobiolecol,2003,46(3):233-246
|
| [24] | 8amannri,ludwigw,schleiferkh.phylogeneticidentificationandinsitudetectionofindividualmicrobialcellswithoutcultivation[j].microbiolmolbiolrev,1995,59(1):143-169
|
| [25] | 9林曦,曾润颖.南极中山站排污口土壤中微生物群落的dgge分析[j].应用与环境生物学报,2008,14(2):253-257[linx,zengry.denaturinggradientgelelectrophoresisanalysisofmicrobialcommunityinsewagecontaminatedsoilfromtheantarcticzhongshanstation[j].chinjapplenvironbiol,2008,14(2):253-
|
| [26] | 10许春红,刘永德,赵继红.pcr-dgge研究carrousel2000氧化沟工艺调试过程中的微生物多样性[j].应用与环境生物学报,2011,17(5):727-730[xuch,liuyd,zhaojh.applicationofpcr-dggetostudymicrobialdiversityduringtheoperatingofcarrousel2000oxidationditch[j].chinjapplenvironbiol,2011,17(5):727-
|
| [27] | 19dorn-ins,h?lzelcs,janket,schwaigerk,balsliemkej,bauerj.pcr-sscp-basedreconstructionoftheoriginalfungalfloraofheat-processedmeatproducts[j].fishsci,2013,162(1):71-81
|
Full-Text
comments powered by
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
WeChat 1538708413
|
|
