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Rela??o entre a atividade de esterases e a resistência de Myzus persicae (Sulz.) (Sternorrhyncha:Aphididae) a pirimicarbe
Furiatti, Rui S.;Lazzari, Sonia M.N.;
Neotropical Entomology , 2003, DOI: 10.1590/S1519-566X2003000400022
Abstract: the objective of this research was to evaluate the relationship between the total activity of carboxylesterase and the resistance of m. persicae populations to pirimicarb. the aphids were collected on potato plants in contenda and piraí do sul, pr and ibicoara, ba, brazil. for the bioassay, five replicates for each concentration were prepared with 10 aphids placed in plastic vials with a fine fabric mesh on the bottom, and dipped into pirimicarb concentrations (%) at 0.0031; 0.0062; 0.0125; 0.025; 0.05, and 0.1%, during 10 seconds, then dried on filter paper. after 1h inside a 25°c chamber, the aphids were examined under a stereomicroscope, recording the number of dead insects. the carboxylesterase activity was assessed by in vitro colorimetric technique. a simple correlation was established between the results of the lc50 bioassay and the frequency of m. persicae resistant variants. according to the absorbance level, the aphids were classified as variants s; s/r1 (s or r1); r1; r1/r2 (r1 or r2); r2 and r3, respectively susceptible; partially resistant, resistant and highly resistant. it was found a significant positive correlation between the lc50 and the frequency of r2 forms and a significant negative correlation between lc50 and the frequency of s and s/r1 forms. the colorimetric data supported the bioassay results, indicating that the bioassay was precise enough to determine either populations with high frequency of susceptible individuals or population with high proportion of resistant and highly resistant individuals.
Determina??o da concentra??o diagnóstica de pirimicarbe para a detec??o de popula??es de Myzus persicae (Sulz.) (Homoptera: Aphididae) com diferentes níveis de esterases
Furiatti, Rui S.;Lázzari, Sonia M. N.;
Anais da Sociedade Entomológica do Brasil , 2000, DOI: 10.1590/S0301-80592000000400013
Abstract: insecticide bioassays associated with colorimetric testes were used to determine the diagnostic concentrations of pirimicarb for insecticide resistance detection in myzus persicae populations from potato crops. the aphids were randomly collected on potato plants of commercial crops in curitiba, piraí do sul and contenda, in paraná state and ibicoara in bahia state, from 1994 to 1996. the lc50 was estimated by submitting the aphids to bioassay with increasing concentrations of pirimicarb. dead and live aphids from the 0.0125; 0.025; 0.05 and 0.1% (g/100ml) insecticide treatments were frozen after one hour of the bioassay and submitted to the evaluation of total esterase by colorimetric assays. mortality was low at 0.0125%, but at 0.1% it reached 100% of the populations; thus, indicating that the diagnostic concentration is within this range. a significant difference was found between mortality caused by 0.025 and 0.05% pirimicarb in the same population, but not between populations collected from different areas. the 0.05% primicarb concentrations caused mortality of nearly 50% of high resistant individuals (r2), 72.0%of the moderately resistant populations (r1) and 89.7% of susceptible ones (s). the determination of this diagnostic concentration is useful for m. persicae resistance management programs because all individuals can be tested at this single appropriate concentration and low resistance frequencies can be detected in the population.
Efeito da aplica??o de pirimicarbe sobre popula??es de campo de Myzus persicae (Sulz.) (Homoptera: Aphididae) com diferentes níveis de esterases
Furiatti, Rui S.;M. N. Lázzari, Sonia;
Anais da Sociedade Entomológica do Brasil , 2000, DOI: 10.1590/S0301-80592000000400014
Abstract: the frequency and intensity of myzus persicae (sulz.) resistance were studied using bioassays and biochemical tests in two potato crops in ibicoara, bahia. both areas received several insecticide applications which failed to control m. persicae. by the end of the crop season, one of the areas received two applications of pirimicarb and the other was not sprayed. six samples of 700 aphids each were randomly collected before and after the pirimicarb applications, with an interval of four days. specimens of m. persicae were also collected from isolated potato plants. at the laboratory, the samples were characterized by the cl50 based on insecticide bioassays and by total esterase activity using colorimetric assays. after the pirimicarb applications, the susceptible (s), the moderate resistant (r1) and mixed s/r1populations presented decreasing resistance frequencies (from 36.6 to 9.9; 12,0 to 7.5 and 11.4 to 5.9%, respectively). on the other hand, high resistant individuals (r2), extremely resistant (r3), and mixed populations of r1/r2 had increasing frequencies (from 17.7 to 36.7%; 2.3 to 9.1%, and 20.0 to 30.8%, respectively). the survival of s individuals was probably due to their wandering behavior on the plants to avoid sprayed areas. all changes in frequency were reflected in the lc50 and in the resistance ratio (rr). in the pirimicarb untreated area, high frequencies of r2, r3 and r1/r2 were observed. high frequency and resistance intensity of m. persicae in areas under intensive insecticide applications can be related to the selection of resistant populations and due to the entrance of winged migrants from spontaneous plants, where the frequency of r2+r3 was 81.4%.
Determina o dos efeitos do meio e estimativa de herdabilidade do peso ao nascer em bovinos da ra a Charolesa
Marco Ant?nio da Rocha,Ivone Yurika Mizubuti,Reginaldo Furiatti
Semina : Ciências Sociais e Humanas , 1983, DOI: 10.5433/
Abstract: Effects of environment and inheritance on weight at birth were studied in a herd located at the city of Bagé in Rio Grande do Sul, BRASIL. The size of the sample included a total of 131 animals and the mathematical model applied to the data included the following variables: year of birth, sire, sex and age of dam. Results showed that only the age of dam was significant (P < 0,05). Heritability was estimated through the method of correlation between paternal half sibs, and 0.30 ± 0,19. Os efeitos do meio e heran a relativos ao peso, ao nascer, foram estudados em um rebanho localizado no município de Bagé, Rio Grande do Sul. O tamanho da amostra em estudo abrangeu um total de 131 animais e o modelo matemático utilizado para o ajuste dos dados incluiu as seguintes variáveis: Ano de nascimento; touro; sexo, e idade da m e. Dos efeitos estudados somente a idade da m e foi significativo (P < 0,05). A herdabilidade calculada através do método de correla o entre meios irm os paternos foi de 0,30 ± 0,19.
Avalia??o de inseticidas no controle de Sitophilus oryzae (L.) (Coleoptera: Curculionidae), e Rhyzopertha dominica (Fab.) (Coleoptera: Bostrichidae) em arroz armazenado
Pinto. Jr., Airton R.;Furiatti, Rui S.;Pereira, Paulo R. V. S.;Lazzari, Flávio A.;
Anais da Sociedade Entomológica do Brasil , 1997, DOI: 10.1590/S0301-80591997000200009
Abstract: the efficacy of insecticides against sitophilus oryzae (l.) (coleoptera: curculionidae) and rhyzopertha dominica (fab.) (coleoptera: bostrichidae) exposed to treated stored rice (oryza sativa) was tested. the insecticides were applied using a propelling air atomizer (5 ml of mixture/kg of rice); for the control only water was used. for each treatment 1.2 kg of stored rice, were treated in plastic bags and stored in raffia bags in a climatized room (25 ± 2oc). fifteen days after treatment, 200 g of the treated rice, from each treatment, were infested with 40 adults of each species. this procedure was repeated every 30 days. the number of dead insects was recorded 15 days after infestations, at 30, 60, 90, 120, 150 and 180 days after the insecticide application. sumigran plus (15 and 20 ml/t) was effective against s. oryzae until 180 days after treatment. sumigran plus (15 and 20 ml/t), sumigran ? + sumidan ? (15 + 15 and 20 + 20 ml/t), sumigran ? + k-obiol ? (15 + 15 ml/t), were effective against r. dominica up to 180 days after treatment.
ácido giberélico, bissulfureto de carbono e ácido 2-4 cloroetil fosf nico e a dormência e produtividade de tubérculos de batata
Ayub Ricardo Antonio,Furiatti Rui Scaramella,Pereira André Belmont,Reghin Marie Y.
Scientia Agricola , 1999,
Abstract: Com o objetivo de avaliar a eficiência do ácido giberélico, bissulfureto de carbono e ácido 2-4 cloroetil-fosf nico (EthrellR) na quebra de dormência e na produ o de batata, cv. "Marijke", foi conduzido um ensaio no período de 1990/ 91 em Piraí do Sul, PR . A superioridade do tratamento com o ácido 2-4-cloroetil-fosf nico em rela o ao ácido giberélico e ao bissulfureto de carbono, aliada a sua facilidade de aplica o, justifica a sua recomenda o com vistas a quebra de dormência de tubérculos do genótipo de batata em estudo, para a regi o de Piraí do Sul, PR, onde a dose de 842 mg.L-1 de EthrellR ocasionou a produ o máxima de 23395 kg/ha.
Avalia??o de inseticidas no controle de Sitophilus oryzae (L.) (Coleoptera: Curculionidae), e Rhyzopertha dominica (Fab.) (Coleoptera: Bostrichidae) em milho armazenado
Pereira, Paulo R.V.S.;Furiatti, Rui S.;Lazzari, Flávio A.;Pinto Jr, Airton R.;
Anais da Sociedade Entomológica do Brasil , 1997, DOI: 10.1590/S0301-80591997000300001
Abstract: the efficacy of insecticides was determined in laboratory against sitophilus oryzae (l.) (coleoptera: curculionidae) and rhyzopertha dominica (fab.) (coleoptera: bostrichidae) exposed to treated shelled corn (zea mays). each treatment, which was composed by 1.2 kg of shelled corn, was treated with 5 ml of mixture/kg of corn using a propelling air atomizer. fifteen days after treatment 200 g of the treated shelled corn, from each treatment, were infested with 40 adult insects of each species and this procedure was repeated every 30 days until 180 days after treatment. the number of dead insects was counted 15 days after each infestation, so the evaluations were made at 30, 60, 90, 120, 150 and 180 days after the insecticide application. after 180 days esfenvalerate + fenitrothion + piperonyl butoxide, esfenvalerate + fenitrothion and fenirothion + deltamethrin + pyperonil butoxide were effective against s. oryzae and r. dominica. fenitrothion was effective against s. oryzae and deltamethrin + piperonyl butoxide against r. dominica. esfenvalerate + pyperonil butoxide were ineffective against s. oryzae and the same was observed with fenitrothion against r. dominica. the use of mixtures of organophosphorus plus pyrethroids were more effective against s. oryzae and r. dominica when compared with these insecticides used alone.
Avalia??o de inseticidas no controle de Sitophilus oryzae (Linnaeus) (Coleoptera, Curculionidae) e Rhyzopertha dominica (Fabricius) (Coleoptera, Bostrichidae) em trigo armazenado
Furiatti, Rui S.;Pereira, Paulo R.V.S.;Pinto Jr., Airton R.;Lazzari, Flávio A.;
Revista Brasileira de Zoologia , 1999, DOI: 10.1590/S0101-81751999000100018
Abstract: the efficiency of insecticides against sitophilus oryzae (linnaeus, 1763) (coleoptera, curculionidae) and rhyzopertha dominica (fabricius, 1792) (coleoptera, bostrichidae) was determined exposing them to treated wheat in laboratory. each plot, which was composed by 1.2 kg of wheat grain, was treated with 5 ml of the concentration/kg of wheat using a propelling air atomizer. fifteen days after treatment 50 g samples of the grain were taken, from each plot, placed in plastic jars into which 10 adult insects of each species were released. this was repeated every 30 days until 165 days after treatment. the number of dead insects was counted 15 days after each infestation thus the evaluations were made at 30, 60, 90, 120, 150 and 180 days after the insecticide application. esfenvalerate+fenitrothion+pyperonil butoxide (0,375+7,5+3,0 and 0,5+10,0+4,0 g a.i./t), fenitrothion+esfen-valerate (7,5+0,375 and 10,0+0,375 g a.i./t), fenitrothion+synergised deltamethrin (7,5+0,375 g a.i./t) and fenitrothion (7,5 g a.i./t) were effective against sitophilus oryzae until 180 days after treatment. esfenvalerate+fenitrothion+pyperonil butoxide (0,5+10,0+4,0 g a.i./t), fenitrothion+esfenvalerate (7,5+0,375 and 10,0+0,375 g a.i./t), fenitrothion+synergised deltamethrin (7,5+0,375 g a.i./t) were effective against r. dominica until 180 days after treatment. synergised deltamethrin (0,375 g a.i./t) was effective against s. oryzae until 150 days and against r. dominica until 120 days after treatment. esfenvalerate (0,5 g a.i./t) was ineffective against s. oryzae and effective until 60 days after treatment against r. dominica. fenitrothion (7,5 g a.i./t) was ineffective against r. dominica. the use of mixtures of organophosphorus plus pyrethroids were more effective against s. oryzae and r. dominica when compared with these insecticides used alone.
ácido giberélico, bissulfureto de carbono e ácido 2-4 cloroetil fosf?nico e a dormência e produtividade de tubérculos de batata
Ayub, Ricardo Antonio;Furiatti, Rui Scaramella;Pereira, André Belmont;Reghin, Marie Y.;Banzatto, David Ariovaldo;Oliveira, Alessandra Valéria de;
Scientia Agricola , 1999, DOI: 10.1590/S0103-90161999000500001
Abstract: in order to evaluate the efficiency of giberellic acid, carbon-disulphide, and 2-4 chloro-ethyl phosphonic acid (ethrellr) on dormancy breaking and yield of potato cv. 'marijke', an essay was conducted at piraí do sul, pr, brazil, during 1990/1991. the superiority of the ethrellr treatment over giberellic acid and carbon-disulphide, and its easy application, justify its use for dormancy breaking of potato tubers, for the studied genotype and location, at the rate of 842 mg.l-1, resulting in a potato yield of 23,395 kg/ha.
Bioatividade de óleos essenciais de sassafrás e eucalipto em cascudinho
Pinto Junior, Airton Rodrigues;Carvalho, Ruy Inácio Neiva de;Netto, Sylvio Pellico;Weber, Saulo Henrique;Souza, Edilaine de;Furiatti, Rui Scaramella;
Ciência Rural , 2010, DOI: 10.1590/S0103-84782010005000026
Abstract: this research aimed to evaluate the sassafras (ocotea odorifera) and eucalyptus (eucalyptus viminalis) essential oils on the control of larvae and adults of lesser mealworm (alphitobius diaperinus), comparing their insecticide potential. the essential oils were produced through water vapor extraction in a semi industrial scale. contaminated paper filter technique was applied to evaluate the insect mortality. in the current experiment twelve concentrations were included for evaluation and among them one control (solvent) in order to settle the mortality rate and lc50 of the two tested essential oils. the larvae were more susceptible then the adults to sassafras essential oil exposure and less susceptible than the adults, when exposed to eucalypt essential oil. larvae as well as adults of a. diaperinus were more susceptible to the sassafras (lc50 adults 0.26ml l-1 and larvae 0.12ml l-1) than the eucalyptus essential oil (lc50 adults 1.37ml l-1 and larvae 1.72ml l-1), by contact intoxication (ocotea odorifera: larvae y=3,3916+1,4699logx; adult y=0,9486+2,8576logx; e eucaliptus viminalis: larvae y=1,3643+1,6254logx; adult y=1,4487+1,6623logx). it is concluded that the essential oils of sassafras and eucalyptus can be used to control lesser mealworm and used as a new integrated strategy for management of this pest.
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