Several microorganisms as bacteria, fungi, yeast and ectoparasites
compose canine ear canal microbiota. These agents can be native or invasive
pathogens that cause clinical otitis. The aim of this study was to associate Mollicutes in external ear canal with
clinical otitis. 41 domestic dogs were examined, and a total of 82 otologic samples
were collected using sterile swabs, placed in a plastic storage tube containing
2 milliliters of sterile phosphate buffer saline (PBS, pH 7.2) and appropriately
labeled. The samples were processed for isolation of Mollicutes using Hayflick’s
medium. The frequency of Mollicutes class in the isolation was 32.9% (27/82).
14.8% (4/27) of the dogs, which were positive for Mollicutes, had external
otitis and 85.2% (23/27) were healthy dogs. The genus Mycoplasma spp. was detected in 7.4% (2/27) by digitonin test. It
was the first isolation of Mollicutes from
the external ear canal in dogs. Although there have been important findings in
this study, the presence of these bacteria cannot indicate the onset and
perpetuation of external otitis in dogs.
Cite this paper
Santos, S. B. D. , Almeida, M. D. S. , Silva, L. T. R. D. , Oliveira, J. M. B. D. , Leal, C. A. D. S. , Junior, J. W. P. and Mota, R. A. (2016). Report of Mollicutes in the Ear Canal of Domestic Dogs in Brazil. Open Access Library Journal, 3, e2790. doi: http://dx.doi.org/10.4236/oalib.1102790.
Merchant, S.R. (2007) Microbiology of the Ear of the Dog and Cat. In:
Gotthelf, L.N., Ed., Small Animal Ear Diseases: An Illustrated Guide,
2nd Edition, Saunders, Philadelphia, 188-200.
Oliveira, L.C., Leite, C.A.L., Brilhante, R.S.N. and Carvalho, C.B.M.
(2008) Comparative
Study of the Microbial Profile from Bilateral Canine Otitis Externa. Canadian Veterinry Journal, 49, 785-788.
Oliveira, L.C., Brilhante, R.S.N., Cunha, A.M.S. and Carvalho, C.B.M.
(2006) Profile of Microorganisms Isolated from Dogs with
Associated Media and Extern Otitis. Brazilian Journal of Veterinary
and Animal Sciences, 58, 1009- 1017.
Sánchez,
R.C., Calle, S.E., Falcón, N.P. and Pinto, C.J. (2011) Bacterial Isolation in Canine Ear Infections and Its
Antimicrobial Susceptibility. Revista de Investigaciones
Veterinárias del Perú, 22,
161-166.
Scartezzini, M., Cordova, D.M., Lima, D.A., Silva, J.C.J. and
Oliveira, S.J. (2011) Bacteriologic Diagnosis of Different Pathologies
of Dogs and Cats, Antimicrobial Susceptibility Tests. Veterinary in Focus, 8, 152-157.
Bugden,
D.L. (2013) Identification
and Antibiotic Susceptibility of Bacterial Isolates from Dogs with Otitis Externa
in Australia. Australian Veterinary Journal, 91, 43-46. http://dx.doi.org/10.1111/avj.12007
Nuttall, T. and Cole, L.K. (2007) Evidence-Based Veterinary Dermatology: A
Systematic Review of Interventions for Treatment of Pseudomonas Otitis
in Dogs. Veterinary Dermatology, 18, 69-77. http://dx.doi.org/10.1111/j.1365-3164.2007.00575.x
Pye, C.C., Yu, A.A.
and Weese, J.S. (2013) Evaluation of Biofilm Production by Pseudomonas aeruginosa from Canine Ears and the Impact of Biofilm on
Antimicrobial Susceptibility in Vitro. Veterinary
Dermatology, 24, 446-e99. http://dx.doi.org/10.1111/vde.12040
Rycroft, A.N., Tsounakou, E. and Chalker, V. (2007) Serological Evidence of Mycoplasma cynosInfection in Canine Infectious Respiratory Disease. Veterinary Microbiology,
120, 358-362. http://dx.doi.org/10.1016/j.vetmic.2006.11.011
Hong, S. and Kim, O. (2012) Molecular Identification of Mycoplasma cynosfrom Laboratory Beagle Dogs with Respiratory Disease. Laboratory
Animal Research, 28, 61-66. http://dx.doi.org/10.5625/lar.2012.28.1.61
Shoetensack, H.M. (1934) Pure Cultivation of Filterable Virus
Isolatedfrom Canine Distemper. The
Kitasato Archives of Experimental Medicine, 11, 227-290.
Spergser, J., Langer, S., Muck, S., Macher,
K., Szostak, M., Rosengarten, R. and Busse,
H.J. (2011) Mycoplasma mucosicanis sp. nov., Isolated from the Mucosa of Dogs. International Journal of Systematic Evololution Microbiology, 61, 716-721. http://dx.doi.org/10.1099/ijs.0.015750-0
Rosendal,
S. (1975) Canine Mycoplasmas: Cultural and Biochemical Studies of Type and
Reference Strains. Acta
Pathologica Microbiologica Scandinavica Section B: Microbiology and Immunology, 83, 457-462.
Rosendal, S. (1978) Canine Mycoplasmas:
Pathogenicity of Mycoplasmas
Associated with Distemper Pneumonia. The
Journal of Infectious Diseases, 138, 203-210. http://dx.doi.org/10.1093/infdis/138.2.203
Rosendal, S. (1982) Canine Mycoplasmas:
Their Ecologic Niche and Role in Disease. Journal of the American Veterinary
Medical Association, 15,
1212-1214.
Almeida, M.S., Santos, S.B., Mota, A.R., Silva,
L.T.R., Silva,
L.B.G. and Mota,
R.A. (2016)
Microbiological Isolation from the Ear Canal of Healthy Dogs and with Otitis
Externa in the Metropolitan Region of Recife, Pernambuco. Pesquisa Veterinária Brasileira, 36, 29-32. http://dx.doi.org/10.1590/S0100-736X2016000100005
Corrales, J.C., Esnal, A., De la Fe, C., Sánchez, A., Assuncao, P., Poveda, J.B. and Contreras,
A. (2007) Contagious Agalactia in Small Ruminants. Small Ruminant Research, 68, 154-166. http://dx.doi.org/10.1016/j.smallrumres.2006.09.010
Santos,
S.B., Nascimento, E.R., Faccini, J.L.H., Barreto, M.L. and Pereira, V.L.A. (2009) Potentially
Pathogenic Mycoplasmas in the External Ear Canal of Clinically Normal Cattle in
Southeast Brazil: First Report. Brazilian
Journal of Microbiology, 40, 455-457. http://dx.doi.org/10.1590/S1517-83822009000300006
Boekhout, T., Guého-Kellermann, E., Mayser, P.
and Velegraki, A. (2010) Malasseziaand the Skin: Science and Clinical Practice. Springer Science and Business Media, Berlin,
108-110.
Edward, D.G. (1955) A
Suggested Classification and Nomenclature
for Organisms of the Pleuropneumonia Group. International
Journal of Systematic and Evolutionary Microbiology, 5, 85-93. http://dx.doi.org/10.1099/0096266x-5-2-85
Armstrong, D., Tully, J.G., Yu, B., Morton, V., Friedman, M.H. and
Steger, L. (1970) Previously Uncharacterized
Mycoplasma Isolates from an Investigation of Canine Pneumonia. Infection and Immunity, 1, 1-7.
Adegboye, D.S.
and Addo, P.B. (1978) Mycoplasmas from Vagina of a Bitch
with Open Cervix Pyometra. Veterinary
Record, 102,
62-63. http://dx.doi.org/10.1136/vr.102.3.62
