Atlantic and Mediterranean
warming-related diseases outbreaks and species shifts recently have been documented.
Evaluated tools of short-term effects on the health or organisms resistance are
necessary to assess and understand mechanisms affecting marine biodiversity.
Until now, climate warming has been studied at the population or community
level. Here we offer a better understanding of such phenomena at the individual
organism level, using anatomic-morphological approaches to interpret effects of
natural physical stressors, according to behavioral patterns. The goal of this
work was to evaluate the sea anemones behavior with temperature variance. This
study takes a method of behavioral observations (morphological and anatomic
parameters, with physiological implications) to identify changes in behavior,
after exposure to the physical stressors temperature (10℃, 15℃, 20℃, 25℃ and 30℃) on temperate sea anemone Actinia
equina over 96 h of
exposure. Other endpoints as condition index and reproduction also assessed.
Behavioral patterns analysis placed the differentially ecological functions in
a wide range of categories including tentacle flexion, tentacle retraction,
column cavitation, peristome depression and oral disc flexion. These parameters
suggest that the “early stress response” (before result on individual death) to
elevated temperature involves essentially all aspects of same chemical
reactions. In this
case we observed receptors functioning and the frequency of open-close oral sea
anemones, tentacles and columns anatomic alterations to detect earlier the
effects of physical stress induction. The superiority of results tested was
that the key species reacted to different temperature ranges in order to demonstrate that species
from different climatic zones could have the same behavioral pattern but have
intrinsic adaptations on each climatic zone. Also some collections of parameters such as: 1) water nutrients
availability, 2)
reproductions rate (number of polyps), 3) survival (condition index) and 4) temperature variations
were significant on behavioral answers.
Cite this paper
Gadelha, J. R. , Jesus, F. , Gomes, P. B. , Osten, J. R. V. , Morgado, F. and Soares, A. M. V. D. M. (2017). Temperature Tolerance Test Exposition with Temperate Sea Anemone Actinia equina, a Climatic and Environmental Changes Simulation. Open Access Library Journal, 4, e3360. doi: http://dx.doi.org/10.4236/oalib.1103360.
Pérez,
S.F., Cook, C.B. and Brooks, W.R. (2001)
The Role of Symbiotic Dinoflagellates in the Temperature-Induced Bleaching
Response of the Subtropical Sea Anemone Aiptasia pallida. Journal of Experimental Marine Biology and
Ecology, 256,
1-14. https://doi.org/10.1016/S0022-0981(00)00282-3
Richier,
S., Sabourault, C., Courtiade, J., Zucchini, N., Allemand, D. and
Furla, P. (2006) Oxidative Stress and Apoptotic Events during Thermal
Stress in the Symbiotic Sea Anemone, Anemonia viridis. FEBS Journal, 273, 4186-4198. https://doi.org/10.1111/j.1742-4658.2006.05414.x
Bhagooli,
R. and Hidaka, M. (2004) Release of Zooxanthellae with Intact Photosynthetic
Activity by the Coral Galaxea fascicularis in Response to High Temperature Stress. Marine
Biology, 145,
329-337. https://doi.org/10.1007/s00227-004-1309-7
Dunn, S.R., Bythell, J.C., Le Tissier, M.D.A.,
Burnett, W.J. and Thomason, J.C. (2002) Programmed Cell Death and Cell
Necrosis Activity during Hyper Thermic Stress-Induced Bleaching of the Symbiotic
Sea Anemone Aiptasia sp. Journal
of Experimental Marine Biology and Ecology, 272, 29-53. https://doi.org/10.1016/S0022-0981(02)00036-9
Muscatine,
L., Grossman, D. and Doino, J. (1991)
Release of Symbiotic Algae by Tropical Sea Anemones and Corals after Cold Shock. Marine Ecology Progress Series, 77.
233-243. https://doi.org/10.3354/meps077233
Gates, R.D., Bahgdasarian, G. and Muscatine,
L. (1992) Temperature Stress Causes Host Cell Detachment in Symbiotic
Cnidarians: Implications for Coral Bleaching. The Biological Bulletin, 182, 324-332. https://doi.org/10.2307/1542252
Di Marco,
T. (2008) Effects of Ultraviolet Radiation on the
Behavior of the Sea Anemone Anthopleura elegantissima. Explorations: An Undergraduate Research Journal, 1-10.
Clark,
E.D. and Kimeldorf, D.J. (1971) Behavioral
Reactions of the Sea Anemone, Anthopleura xanthogrammica, to Ultraviolet and
Visible Radiations. Radiation Research,
45, 166-175. https://doi.org/10.2307/3573089
Walsh,
P.J. and Somero, G.N. (1981) Temperature Adaptation in Sea Anemones: Physiological
and Biochemical Variability in Geographically Separate Populations of Metridium
senile. Marine Biology, 62,
25-34. https://doi.org/10.1007/BF00396948
Gadelha,
J.R., Morgado, F.
and Soares, A.M.V.M. (2013) Histology
and Histochemistry of Sea Anemones in Environmental Contamination Studies. Microscopy
and Microanalysis, 19, 57-58. https://doi.org/10.1017/S1431927613000901
Bae, M.-J.
and Park, Y.-S.
(2014)
Biological Early Warning System Based on the Responses of Aquatic Organisms to Disturbances:
A Review. Science of the Total Environment, 466-467,
635-649. https://doi.org/10.1016/j.scitotenv.2013.07.075
Moreira,
S.M., Moreira-Santos, M., Ribeiro, R. and Guilhermino, L. (2005) The “Coral
Bulker” Fuel Oil Spill on the
North Coast of Portugal: Spatial and Temporal Biomarker Responses in Mytilus galloprovincialis. Ecotoxicology, 13, 619-630. https://doi.org/10.1007/s10646-003-4422-3
Zelnio, K.A., Rodriguez, E. and Daly, M. (2009) Hexacorals (Anthozoa:
Actiniaria, Zoanthidea) from Hydrothermal Vents in the Southwestern Pacific. Marine Biology Research, 5,
547-571. https://doi.org/10.1080/17451000902729662
Scott,
A. and Harrison, P.L. (2009)
Gametogenic and Reproductive Cycles of the Sea Anemone, Mytilus galloprovincialis. Marine Biology, 156, 1659-1671. https://doi.org/10.1007/s00227-009-1201-6
Gadelha, J.R.,
Ferreira, V., Abreu, S.N., Soares, A.M.V.M. and Morgado, F. (2010) Actinia
equina Bioassays Adopting Techniques from Acclimatizing in a Flow-Through System. SETAC Europe Meeting, Sevilla, 23-27 May
2010, 196.
Gadelha, J.R., Asensio,
A.R., Gonzales, I., Aboal, J.R., Carballeira, A., Vieira, L.R., Guilhermino, L.,
Abreu, S.N., Rendón von Osten, J., Morgado, F. and Soares, A.M.V.M. (2011) Differential Accumulation of Metals and Changes in Cholinesterases
and 15N Levels in the Sea Anemone Anemoniasulcata (Cnidaria) as a Function of Aquaculture
Effluent Gradient Exposition. SETAC
Europe Meeting, Milan, 15-19 May 2011,
152.
Gadelha, J.R., Morgado, F., Faustino,
A. and Soares, A.M.V.M. (2012) Histological and Structural Analysis of Actinia
equina L. (Cnidaria: Anthozoa). Microscopy
and Microanalysis, 18,
61-62. https://doi.org/10.1017/S1431927612012962
Jordi,
P. and Green, A.J. (2009)
New Perspectives for Estimating Body Condition from Mass/Length Data: The
Scaled Mass Index as an Alternative Method. Oikos, 118, 1883-1891. https://doi.org/10.1111/j.1600-0706.2009.17643.x
Jeffrey, S.W. and Humphrey, G.F. (1975) New Spectrophotometric
Equations for Determining Chlorophylls a, b, c and c2 in Higher Plants, Algae and Natural Phytoplankton. Biochemie und Physiologie der Pflanzen, 167,
191-194.
Chomsky,
O., Kamenir, Y., Hyams, M., Dubinsky, Z. and
Chadwick-Furman, N.E. (2004) Effects of Temperature on Growth
Rate and Body Size in the Mediterranean Sea Anemone Actinia equina. Journal of Experimental Marine Biology and Ecology, 313, 63-73. https://doi.org/10.1016/j.jembe.2004.07.017
Chomsky,
O., Douek, J., Chadwick, N.E., Dubinsky, Z., Mina, B.R. and Goodm, E. (2009) Biological and Population-Genetic
Aspects of the Sea Anemone Actinia equina (Cnidaria: Anthozoa) along the
Mediterranean Coast of Israel. Journal of
Experimental Marine Biology and Ecology, 375, 16-20. https://doi.org/10.1016/j.jembe.2009.04.017
Dunn,
S.R., Thomason, J.C., Le Tissier, M.D.A. and Bythell, J.C. (2004) Heat Stress Induces Different Forms of Cell Death in Sea Anemones and Their
Endosymbiotic Algae Depending on Temperature and Duration. Cell Death
and Differentiation, 11, 1213-1222. https://doi.org/10.1038/sj.cdd.4401484
Chomsky,
O., Kamenir, Y., Hyams, M., Dubinsky, Z. and
Chadwick-Furman, N.E. (2004) Effects of Feeding Regime on
Growth Rate in the Mediterranean Sea Anemone Actinia equina (Linnaeus). Journal
of Experimental Marine Biology and Ecology, 299,
217-229. https://doi.org/10.1016/j.jembe.2003.09.009
Beitinger,
T.L. and McCauley, R.W. (1990)
Whole-Animal Physiological Processes for the Assessment of Stress in Fishes. Journal of Great Lakes Research, 16,
542-575. https://doi.org/10.1016/S0380-1330(90)71445-1
Dell’Omo,
G., Pleskacheva, M., Wolfer, D.P., Lipp, H.-P.
and Shore, R.F. (2003)
Comparative Effects of Exposure to an Organophosphate Pesticide on Locomotor Activity
of Laboratory Mice and Five Species of Wild Rodents. Bulletin of Environmental Contamination and Toxicology, 70, 138-145. https://doi.org/10.1007/s00128-002-0167-6
Scott,
G.R. and Sloman, K.A. (2004)
The Effects of Environmental Pollutants on Complex Fish Behavior: Integrating
Behavioral and Physiological Indicators of Toxicity. Aquatic Toxicology, 68, 369-392. https://doi.org/10.1016/j.aquatox.2004.03.016
Lagadic,
L., Caquet, T. and Ramade, F. (1994)
The Role of Biomarkers in Environmental Assessment Invertebrate Populations and
Communities. Ecotoxicology, 3,
193-208. https://doi.org/10.1007/BF00117084