The Comparison of Area under the Curve of Serum Estradiol during Controlled Ovarian Stimulation between Long Gonadotrophin Releasing Hormone Agonist Protocol and Short Gonadotrophin Antagonist Protocol in Invitro Fertilization
Background: during stimulation,
estradiol levels represent the dynamic hormonal interaction of follicle
development. Some studies have been done to evaluate the influence of day-hCG
estradiol assay and IVF outcome. However, this assay does not reflect the total
exposure of estradiol throughout stimulation. Hence the concept of calculating
the area under the curve (AUC) of estradiol during ovarian stimulation is
thought to be a better parameter to evaluate total exposure of estradiol to the
entire process of IVF. Methods: a retrospective analysis of data from a fertility
center in a tertiary hospital. Between 2011-2016, there were long GnRH agonist
cycles and short GnRH antagonist cycles eligible for analysis. Data including
subject’s characteristics, the level of estradiol on day-2, day-6, and day-hCG
during controlled ovarian stimulation were obtained. Those data were plotted,
and area under the curve of each subject was calculated. Statistical analysis
was done using student t-test while p < 0.05 was considered significant. Results:
there were no significant differences in subject’s age (33.4 vs. 34.2 years, p = 0.12), day-3 of menstrual cycle FSH level (7.1 vs. 7.3 mIU/mL, p = 0.64), and day-3 estradiol level (35.1 vs. 36.5 pg/mL, p = 0.61). The estradiol level on day-5 of COS was
324.9 (273.1) pg/mL and 363.3 (293) pg/mL (p = 0.36), and at the day of hCG
administration was
2461.8 (2283.7) ng/mL and 2558.2 (2043) ng/mL (p = 0.76). Estradiol
area under the curve for long protocol and short protocol was 9205.2 (7683.7) pg/mL and 9732.9
(6893.9) pg/mL respectively (p = 0.63). Conclusion: our results
indicate that there are no significant differences in the area under the curve
of estradiol between two protocols.
Cite this paper
Bayuaji, H. , Permadi, W. , Sastramihardja, H. S. and Nataprawira, H. M. N. (2017). The Comparison of Area under the Curve of Serum Estradiol during Controlled Ovarian Stimulation between Long Gonadotrophin Releasing Hormone Agonist Protocol and Short Gonadotrophin Antagonist Protocol in Invitro Fertilization. Open Access Library Journal, 4, e3832. doi: http://dx.doi.org/10.4236/oalib.1103832.
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