The bioactive compounds in
methanol extract of Xylopia aethiopica fruits were determined using a combination of gas chromatography and mass
spectrometry (GC-MS). The compound identification was based on the molecular
structure, molecular mass and calculated fragments. Interpretation on mass
spectrum of GC-MS analyzer (Hewlett Packard GC-MS system) was done using the
database of National Institute Standard and Technology (NIST). The results
showed different peaks representing the presence of about 58 bioactive
compounds which are mainly carboxylic acids, esters, phenolic compounds and
fatty acids. The most abundant bioactive compounds detected were 2,4,6-octatriene (2.74%),
cyclohexanemethanol (2.57%), kaurene (3.59%), 9,12-octadecadienoic acid
(5.63%), 1,6-cyclodecadiene (10.81%), terpineol (3.22%), cyclohexene (3.32%), copaene
(3.04%), 1,6-cyclodecadiene (5.53%), 1-hexadecyne (5.63%) and silane (4.63%).
The presence of these bioactive components suggests that the extract is of
great pharmaceutical value.
Cite this paper
Okagu, I. U. , Ngwu, U. E. and Odenigbo, C. J. (2018). Bioactive Constituents of Methanol Extract of Xylopia aethiopica (UDA) Fruits from Nsukka, Enugu State, Nigeria. Open Access Library Journal, 5, e4230. doi: http://dx.doi.org/10.4236/oalib.1104230.
Ashokkumar, R. and Ramaswamy, M. (2014) Phytochemical Screening by FTIR Spectroscopic Analysis of Leaf Extracts of Selected Indian Medicinal Plants. International Journal of Current Microbiology and Applied Sciences, 3, 395-406.
Murugan, M. and Mohan, V.R. (2014) Phytochemical, FT-IR and Antibacterial Activity of Whole Plant Extract of Aerva lanata (L.) Juss. Ex. Schult. Journal of Medicinal Plants Studies, 2, 51-57.
Ashish, C., Manish, K.G. and Priyanka, C. (2014) GC-MS Technique and Its Analytical Applications in Science and Technology. Journal of Analytical and Bioanalytical Techniques, 5, 6-10.
Eze, K.N. (2012) Antifertility Effects of Ethanolic Extract of Xylopia aethiopica on Male Reproductive Organ of Wistar Rats. American Journal of Medicine and Medical Sciences, 2, 12-15.
Fetse, J.P., Kofie, W. and Adosraku, R.K. (2016) Ethnopharmacological Importance of Xylopia aethiopica (dunal) a. Rich (Annonaceae)—A Review. British Journal of Pharmaceutical Research, 11, 1-21. https://doi.org/10.9734/BJPR/2016/24746
Ezekwesili, C.N., Nwodo, O.F.C., Eneh, F.U. and Ogbunugafor, H.A. (2010) Investigation of the Chemical Composition and Biological Activity of Xylopia aethiopica Dunal (Annonacae). African Journal of Biotechnology, 9, 7352-7356.
Onyebuagu, P.C., Kiridi, K. and Aloamaka, C.P. (2014) Effect of Dietary Xylopia aethiopica on the Gonads of Male Wistar Rats. International Journal of Herbs and Pharmacological Research, 3, 40-45.
Hassan, A.M., Almagboul, A.Z. and Kabbashi, A.S. (2016) Chemical Compositions and Cytotoxic Activity of Fruits Essential Oil from Xylopia aethiopica (Dunal) A. Rich. International Journal of Multidisciplinary Research and Development, 3, 394-399.
Erhirhie, E.O. and Moke, G.E. (2014) Xylopia aethiopica: A Review of Its Ethnomedicinal, Chemical and Pharmacological Properties. American Journal of Pharmtech Research, 4, 22-37.
Uzodike, E.B. and Onuoha, I.N. (2010) The Effect of Xylopia aethiopica (Uda) on Intraocular Pressure. Journal of the Nigerian Optometric Association, 16, 21-25. https://doi.org/10.4314/jnoa.v16i1.56629
Chris-Ozoko, L.E., Ekundina, V. and Winiki, C. (2015) Histomorphological Effects of Xylopia aethiopica on the Liver and Kidney of Albino Wistar Rats. Scholars Academic Journal of Biosciences, 3, 150-154.
Elhassan, I.A. and Ayoub, S.M.H. (2014) Effects of Geographic Location on Essential Oil Content and Composition of Xylopia aethiopica. American Journal of Research Com-munication, 2, 251-261.
Noudjou, F., Kouninki, H., Hance, T., Haubruge, E., Ngamo, L.S.T. and Maponmestsem, P.M. (2007) Composition of Xylopia aethiopica (Dunal) A. Rich Essential Oils from Cameroon and Identification of a Minor Diterpene: [Ent-13-epimanoyl oxide]. Biotechnology and Agronomy of Social Environment, 11, 193-199.
Konan, N.D., Bosson, A.K., Mamyrbekova-Bekro, J.A., Jean, N. and Bekro, Y.A. (2009) Chemical Composition and Antioxidant Activities of Essential Oil of Xylopia aethiopica (Dunal) A. Rich. European Journal of Scientific Research, 2, 311-318.
Olonisakin, A.M., Oladimeji, O. and Lajide, L. (2007) Composition and Antibacterial Activity of Steam Distilled Oils of Xylopia aethiopica and Syzigium aromaticum. Journal of Engineering and Applied Sciences, 2, 236-240.
Koba, K., Sanda, K., Raynaud, C., Guyon, C., Chaumont, J.P. and Nicod, L. (2008) Chemical Composition and in Vitro Cytotoxic Activity of Xylopia aethiopica (Dun) A. Rich. (Annonaceae) Fruit Essential Oil from Togo. Journal of Essential Oil Research, 20, 354-357. https://doi.org/10.1080/10412905.2008.9700029
EL-Kamali, H.H. and Adam, H.O. (2009) Aromatic Plants from the Sudan: Part II. Chemical Composition of the Essential Oil of Xylopia aethiopica (Dunal) A. Rich. Existence of Chemotype Species. Advances in Natural and Applied Sciences, 3, 166-169.
Apenteng, J.A., Ogundeyi, M., Oppong, E.E., Osei-Asare, C. and Brookman-Amissah, M.G. (2016) In Vitro Anti-Infective and Antioxidant Activity of Xylopia aethiopica [Dun.] A. Rich: A Comparison of the Fruits and Leaves Extracts. Journal of Medicinal Plants Studies, 4, 24-29.
Sujayil, T.K. and Dhanaraj, T.S. (2016) Determination of Bioactive Compounds in Evolvulus alsinoides Leaf Extract using GC-MS Technique. Research Journal of Life Sciences, Bioinformatics, Pharmaceutical and Chemical Sciences, 2, 34-38.
Gnanavel, V. and Saral, A.M. (2013) GC-MS Analysis of Petroleum Ether and Ethanol Leaf Extracts from Abrus precatorius linn. International Journal of Pharma and Bioscience, 4, 37-44.
Santhosh, K.S., Samydurai, P., Ramakrishnan, R. and Nagarajan, N. (2014) Gas Chromatography and Mass Spectrometry Analysis of Bioactive Constituents of Adiantum Capillus Veneris L. International Journal of Pharmacy and Pharmaceutical Sciences, 6, 60-63.
Mamza, U.T., Sodipo, O.A. and Khan, I.Z. (2012) Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Bioactive Components of Phyllanthus amarus Leaves. International Research Journal of Plant Science, 3, 208-215.
Telascrea, M., de Araújo, C.C., Marques, M.O.M., Facanali, R., de Moraes, P.L.R. and Cavalheiro, A.J. (2007) Essential Oil Leaves of Cryptocaryam andioccana Meisner (Lauraceae): Composition and Intraspecific Chemical Variability. Biochemical System and Ecology, 35, 222-232. https://doi.org/10.1016/j.bse.2006.09.015
Kiran, S.R. and Devi, P.S. (2007) Evaluation of Mosquitocidal Activity of Essential Oil and Sesquiterpenes from Leaves of Chloroxylon swietenia DC. Parasitology Research, 101, 413-418. https://doi.org/10.1007/s00436-007-0485-z
Hamadan, D.I., Abdulla, R.H., Mohamed, M.E. and El-Shazly, A.M. (2013) Chemical Composition and Biological Activity of Essential Oils of Cleopatra mandarin (Citrus reshni) Cultivated in Egypt. Journal of Pharmacognosy and Phytotherapy, 5, 83-89.
Birkett, M.A., Al Abassi, S., Krober, T., Chamberlain, K., Hooper, A.M., Guerin, P.M., Pettersson, J., Pickett, J.A., Slade, R. and Wadhams, L.J. (2008) Antiectoparasitic Activity of the Gum Resin, Gum Haggar, from the East Africa Plant, Commiphor aholtziana. Phytochemical, 69, 1710-1715. https://doi.org/10.1016/j.phytochem.2008.02.017
Prakasia, P.P. and Nair, A.S. (2015) Chemical Fingerprint of Essential Oil Components from Fresh Leaves of Glycosmis pentaphylla (Retz.). Correan Pharmaceutical Innovation Journal, 3, 50-56.
Soh, D., Nkwengoua, E., Ngantchou, I., Nyasse, B., Denier, C. and Hannaert, V. (2013) Xylopioxyde and Other Bioactive Kaurane-Diterpenes from Xylopia aethiopica Dunal (Annonaceae). Journal of Applied Pharmaceutical Science, 3, 13-19.
Enemchukwu, B.N., Erimujor, S.O. and Ubaoji, K.I. (2014) Phytochemical Screening and Biochemical Effects of Aqueous Seed Extract of Xylopia aethiopica (uda) on Selected Haematological Indices in Male Wistar Albino Rats. The Bioscientist, 2, 103-109.
