%0 Journal Article %T Screening of Single Nucleotide Polymorphism in CD95 (APO-1/FAS) Promoter Region (G-1377A) in Children with Acute Leukemia %A Dilara Fatma Akin Bali %A Didem Torun Ozkan %A Ahmet Emin Kurekci %A Nejat Akar %J Open Access Library Journal %V 5 %N 3 %P 1-7 %@ 2333-9721 %D 2018 %I Open Access Library %R 10.4236/oalib.1104385 %X
CD95 is a cell surface receptor involved in apoptotic signal transmission. Deregulation of this pathway results in down regulation of apoptosis and subsequent persistence of a malignant clone. A single nucleotide polymorphism (SNP) resulting in guanine-to-adenine (G > A) transition in the CD95 promoter region (position 1377) is thought to reduce stimulatory protein 1 (SP1) transcription factor binding and decrease CD95 expression. The purpose of this study was to examine a genetic polymorphism in the core promoter of CD95 is associated with the frequency. G-1377A in the CD95 promoter region was genotyped by polymerase chain reaction and restriction endonuclease analysis and sequenced of the CD95 gene (Beckman Coulter, USA). Among 146 patients, CD95 G-1377A (rs2234767) SNP carriers frequencies has been identified as 25% GA (n:37) and AA 4% (n:6), respectively. This polymorphism of the distribution of the CD95 gene in acute leukemic children will be a guide for future studies.
%K Acute Lymphoblastic Leukemia %K Acute Myeloblastic Leukemia %K CD 95 Polymorphism %U http://www.oalib.com/paper/5293127