Biochemical metabolites are implicated in preeclampsia, which include elevated plasma concentration of Triglycerides (TG), Low Density Lipoprotein Cholesterol (LDLC), total lipids and reduced High Density Lipoprotein Cholesterol (HDLC) concentrations and significant reductions in fasting plasma glucose, and serum insulin homeostasis model of assessment. Vitamin D supplementation seems to be a possible target for intervention and possible improved pregnancy outcomes in preeclampsia. Therefore, this study aimed at determining the effects of Vitamin D supplementation on the Lipid Profile, and random plasma glucose levels of preeclamptic women in Ibadan, Nigeria. This interventional study was carried out at the Antenatal clinic of Obstetrics and Gynecology Departments of University College Hospital Ibadan and Adeoyo Maternity Clinic, Yemetu, Ibadan, Nigeria. 120 pregnant women aged 18 to 35 were recruited; 40 normotensive pregnant women served as control; 40 randomly chosen preeclamptic women were placed on oral vitamin D supplement of 1000 IU/day for 8 weeks and 40 preeclamptic women were given no supplement. Blood samples were taken at study baseline (22 weeks), after 8 weeks of supplementation (3^rd trimester) and 3 to 5 days postpartum in all groups to quantify serum vitamin D, random glucose and Lipid profile. Vitamin D was quantified by Enzyme Linked Immunosorbent Assay (ELISA), glucose status by glucose oxidase method and lipid profile by standard methods. Statistical analysis was performed using Statistical Package for Social Sciences (SPSS) soft version 17.0 and subjected to One way analysis of Variance (ANOVA) and Student¡¯s t test. Results showed a significant increase (p < 0.05) in levels of TG, LDLC, random plasma glucose and a decrease (p < 0.05) in HDLC levels in the preeclamptic groups when compared with normotensive women in second and third trimester. After vitamin D supplementation, there was a significant reduction (p < 0.05) in Total Cholesterol (TC), TG, LDLC levels and an increase in HDLC (p < 0.05) (4.49 ¡À 0.38, 1.51 ¡À 0.22, 3.45 ¡À 0.57, 1.87 ¡À 0.29) vs. (5.96 ¡À 0.49, 2.30 ¡À 0.20, 5.90 ¡À 0.36, 1.07 ¡À 0.28) respectively. Random plasma glucose level was reduced (5.7 ¡À 0.5 vs. 6.01 ¡À 0.43), though not statistically significant (p > 0.05). Vitamin D supplementation is important in ameliorating dyslipidemia associated with preeclampsia.