The effects of biofungicide formula containing the
biocontrol agent Trichoderma harzianum (Jn14) as wettable powder in
combination with the fungicides pyrimethanil and a mixture of cyprodinil and
flydioxonil on Botrytis cinerea of strawberry in vitro, in
vivo and in greenhouse were studied. The pathogen B. cinerea was
more affected in vitro by low concentrations of the fungicides tested
above 0.4 μg·ml-1 than the bioagent T. harzianum (Jn14). The later was almost insensitive to pyrimethanil. In
the same direction, gray mould disease severity on strawberry detached
leaves and whole plants was reduced up to 89% by the tested fungicides,
compared to the control, while the application of T. harzianum (Jn14)
alone reduced disease severity up to 45% on strawberry detached leaves,
compared to the control. In the integrated control approach, the combination of T. harzianum (Jn14) with higher concentrations of the tested fungicides
(ED50) completely inhibited strawberry gray mould disease severity
in pots and the greenhouse.
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