Development of novel biomaterials and its practical
application have been the subject of much research in the field of scaffolds
for tissue engineering, providing the success of producing scaffolds
biomaterials that facilitate tissue growth and provide structure support for
cells. Due to its biocompatibility and biodegradability, chitosan has been the
focus of several researches in recent years to be applied in the biomedical
field and chemical modifications of chitosan through crosslinks can produce
materials with a wide variety of properties. The objective of this study was to
obtain and characterize, chemically and biologically, ionically crosslinked
chitosan membranes. Chitosan membranes were
prepared by solvent evaporation and the crosslinks were introduced by reaction
with sulfuric acid solution. The cross-linked membranes were characterized by
Fourier transform infrared (FTIR), X-ray diffraction (XRD), Scanning Electron
Microscopy (SEM), X-ray Dispersive Energy Spectroscopy (EDX) and contact angle
measurements. The biological assays of the membranes were performed with NIH
3T3 cells in two steps: evaluation of cytotoxicity by indirect contact of the
cells with the extracts of the chitosan membranes and finally with the direct
contact of the cells on the filaments of chitosan to obtain adhesion and cell
proliferation information. Non-crosslinked membranes of chitosan were
used as controls for all assays. With the physicochemical tests, it was
possible to observe an effective crosslinking reaction in chitosan. Biological
assays have revealed that the membranes are non-cytotoxic but must still be
modified to provide cell adhesion and proliferation.
Cite this paper
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