Rice is the staple food crop for a large part of the human population in the world today. Rice blast is by far the most important disease of the many diseases that attack rice caused by Magnaporthe oryzae oryzae (MoO). Failures of entire rice crops have resulted directly from rice blast epidemics. Trichoderma harzianum the well-known antagonistic fungus is widely used in agriculture as bio-fungicide. In the present study five different designs of in vitro dual culture techniques were evaluated on Potato Dextrose Agar (PDA) to find out the most suitable technique to interaction study between MoO and T. harzianum THR 4. The design where four 5.0 mm mycelia discs of T. harzianum THR 4 were set on the periphery of the petridish surrounding one disc of MoO on the center of the petridish in dual culture gave most satisfactory result with 92% growth inhibition of MoO in compare to control plate of MoO.
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Nazifa, Z. , Aminuzzaman, F. M. , Laila, L. and Ashrafi, M. L. (2021). Comparative in Vitro Efficacy Assessment Methods of a Bioagent Trichoderma harzianum THR 4 against Rice Blast Pathogen Magnaporthe oryzae oryzae. Open Access Library Journal, 8, e7510. doi: http://dx.doi.org/10.4236/oalib.1107510.
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