%0 Journal Article
%T Trypanocidal activity of human plasma on Trypanosoma evansi in mice
%A Da Silva
%A Aleksandro Schafer
%A Duck
%A Marcos Rafael Kroeker
%A Fanfa
%A Vinicius da Rosa
%A Otto
%A Mateus Anderson
%A Nunes
%A Jo£żo Tomaz Schmitt
%A Tonin
%A Alexandre Alberto
%A Jaques
%A Jeandre Augusto
%A Paim
%A Francine Chimelo
%A Duarte
%A Marta Maria Medeiros Frescura
%A Monteiro
%A Silvia Gonzalez
%J Revista Brasileira de Parasitologia Veterin¨˘ria
%D 2012
%I Scientific Electronic Library Online
%R 10.1590/S1984-29612012000100011
%X this study aimed to test an alternative protocol with human plasma to control trypanosoma evansi infection in mice. plasma from an apparently 27-year-old healthy male, blood type a+, was used in the study. a concentration of 100 mg.dl-1 apolipoprotein l1 (apol1) was detected in the plasma. forty mice were divided into four groups with 10 animals each. group a comprised uninfected animals. mice from groups b, c and d were inoculated with a t. evansi isolate. group b was used as a positive control. at three days post-infection (dpi), the mice were administered intraperitoneally with human plasma. a single dose of 0.2 ml plasma was given to those in group c. the mice from group d were administered five doses of 0.2 ml plasma with a 24 hours interval between the doses. group b showed high increasing parasitemia that led to their death within 5 dpi. both treatments eliminated parasites from the blood and increased the longevity of animals. an efficacy of 50 (group c) and 80% (group d) of human plasma trypanocidal activity was found using pcr. this therapeutic success was likely achieved in the group d due to their higher levels of apol1 compared with group c.
%K trypanosoma evansi
%K treatment
%K human plasma
%K apol1.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1984-29612012000100011&lng=en&nrm=iso&tlng=en