%0 Journal Article
%T Efecto de la suplementaci¨®n con L-ciste¨ªna en la maduraci¨®n in vitro de ovocitos bovinos
%A Hern¨¢ndez-Fern¨¢ndez
%A Adirmo
%A Hern¨¢ndez-Fonseca
%A Hugo
%J Revista Cient¨ªfica
%D 2010
%I Scientific Electronic Library Online
%X in vitro fertilization (ivf) is now a routine technique in a number of biotechnology research laboratories worldwide. ivf is used as a tool for the study of various aspects related to oocytes maturation, fertilization and early embryo development. l-cysteine constitutes an external substrate required for synthesis of glutathione in the maturation of cattle oocytes. in order to study the effect of l-cysteine supplementation on in vitro maturation of cattle oocytes, ovaries were obtained from crossbred cows culled at a local slaughterhouse. three different in vitro maturation treatments were utilized. each treatment had a different concentrations of l-cysteine: t1: 0 mm, t2: 0.1 mm and t3: 1.0 mm. approximately 400 cumulus oocyte complexes (cocs) were matured in wells of 500 ¨¬l of maturation medium (tcm-199), with 5% co2 at 38.5¡ãc and saturated relative humidity. data were analyzed using analysis of variance (anova). there were no significant differences between treatments in term of oocytes that arrived to metafase ii (mii, t1: 72.77%, t2: 67.47% and t3: 70.43%). results indicate that supplementation with l-cysteine during maturation did not possitively affect the percentage of bovine oocytes that reached the m ii state. nonetheless further research should be carried out to determine the effect of l-cysteine supplementation during maturation, on fertilization and on embryonic development in vitro.
%K ivm
%K bovine
%K l-cysteine
%K oocyte.
%U http://www.scielo.org.ve/scielo.php?script=sci_abstract&pid=S0798-22592010000300008&lng=en&nrm=iso&tlng=en