%0 Journal Article
%T Multiplicaci¨®n del clon de banano ¡®FHIA-18' (AAAB) en Sistema de Inmersi¨®n Temporal
%A Basail P¨¦rez
%A Milagros
%A Medero Vega
%A Victor
%A Ventura Mart¨ªn
%A Jos¨¦ de La Caridad
%A Otero G¨¢lvez
%A Eneida
%A Torres Delgado
%A Marlenys
%A L¨®pez Torres
%A Jorge
%A Cabrera Jova
%A Manuel
%A Santos Pino
%A Arletys
%A Rayas Cabrera
%A Aym¨¦
%A Bauta Toledo
%A Maricel
%A Beovidez Garc¨ªa
%A Yoel
%J Revista Colombiana de Biotecnolog¨ªa
%D 2012
%I Universidad Nacional de Colombia
%X in order to develop a protocol for multiplication of banana clone 'fhia-18' (aaab) in temporary immersion systems, the following working objectives were defined: to determine the effect of immersion time (5, 10 and 15 minutes) and frequency (3, 6 and 8 hours per day), as well as, the influence of different combinations of growth regulators (2,0; 3,0 and 4,0 mg.l-1 de 6-bap and 2,0; 2,5; 3,0; 3,5 and 4,0 mg.l-1 de 6 aia), the volume effect of culture medium plants (20, 30, 40 and 50 ml/explant) and explants density per culture flask (30, 50, 70 y 90 explants/flask) to increase the multiplication coefficient. with 10 minutes immersion time and an immersion frequency every three hours, the best results were achieved in relation to the number of explants obtained. with this immersion time and frequency, explants showed the highest pseudostem diameter. seventy explants were inoculated in each 10,0 l culture flask. the highest productivity at the multiplication phase was achieved with a culture medium renewal of 2800 ml (40 ml/explants), and a 21 day culture time. in addition to using ms salts supplemented with 3.5 mg.l-1 of 6-bap, 1.30 mg.l-1 of iaa, 10.0 mg.l-1 ascorbic acid, the unnecessary growth of stems and leaves of shoots in the multiplication phase was reduced and; therefore, a greater number per explant.
%K coefficient
%K explants density
%K time
%K frequency
%K culture medium volume.
%U http://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S0123-34752012000100002&lng=en&nrm=iso&tlng=en