%0 Journal Article
%T Ligadura de la arteria coronaria descendente anterior izquierda en ratas: T¨¦cnica quir¨²rgica
%A Olivares R
%A Gabriel
%A Mor¨¢n V
%A Sergio
%A Palacios M
%A Cristian
%A Apablaza E
%A Felipe
%A Godoy J
%A Iv¨¢n
%A Zalaquett S
%A Ricardo
%A Ocaranza J
%A Mar¨ªa Paz
%J Revista chilena de cirug¨ªa
%D 2012
%I Scientific Electronic Library Online
%R 10.4067/S0718-40262012000100003
%X background: an experimental model for ligation of the left anterior descending coronary artery (ada) in rats has been commonly used in research into ischemic cardiopathy. however, this procedure is very difficult, with a high mortality rate. there is little information in the literature regarding its technical aspects. our objective was to describe a surgical technique for ada ligation in rats. material and method: fifty-one sprague-dawley male rats weighting 230 ¡À 20 gr were anaesthetized and connected to mechanical ventilation. through a left-side thoracothomy we ligated the ada 2 mm below the junction of the pulmonary artery and the left atrial appendage. the next day we performed transthoracic echocardiography to confirm our results. results: the mortality rate was 7.8% (4 out of 51). the main cause of death was hemorrhage during surgery. the average time for the procedure was 45 ¡À 5 min. we confirmed hypokinesia of the anterior lateral wall in 100% of the surviving rats. discussion: this procedure was reproducible and safe, with less than 10% mortality.
%K ligation
%K coronary vessels
%K rats
%K animal experimentation
%K heart surgical procedure.
%U http://www.scielo.cl/scielo.php?script=sci_abstract&pid=S0718-40262012000100003&lng=en&nrm=iso&tlng=en