%0 Journal Article
%T Efecto card¨ªaco del extracto de epitelio pigmentado de la retina (melanina), del globo ocular de embri¨®n de pollo £¿in vivo£¿ e £¿in vitro£¿
%A ¨¢lvarez
%A Marco A
%A Ercolino
%A Jos¨¦ M
%J Revista de la Facultad de Medicina
%D 2009
%I Scientific Electronic Library Online
%X melanin and its involvement in the genesis of certain cardiac diseases, has recently been revised. however, the expression of functional and cellular effects on the heart has not been clearly established. in this paper we made use of the extract of the retinal pigmented epithelium of the eyeball of the chick embryo, containing melanin, to study "in vivo" the pattern of contraction of the heart and the heart rate by videocardiograma semi-invasive method in embryos of 3 £¿ -4 £¿ days of incubation, and in vitro, their effect on the pattern of mitochondrial using hanging drop method, to primary culture of cardiac myoblasts, incubated with the cationic fluorochrome, 3,3-dimetiloxicabonocianida (dioc1 [3]). the treatment promoted a malfunction of the peristaltic contraction of the embryonic heart, with an increase in atrial filling and reduced ventricular filling during diastole. we determined a significant reduction in heart rate of 18.73 %, after an hour of treatment. the population of myoblasts showed a diffuse pattern of fluorescence, mitochondria were rounded and the cytoplasm showed the presence of blebs at the surface unlike controls with a uniform pattern of green fluorescence emitted by the elongated shape of mitochondria. the results suggested that the extract of retinal pigmented epithelium, melanin containing, alters the peristaltic contraction and decrease the heart rate in experimental model of chick embryo, together with mitochondrial damage, probably linked to the activation of a process of cell death mediated by factors apoptotic mitochondria, which could be associated with such effects.
%K retinal pigmented epithelium
%K melanin
%K cardiotoxicity
%K videocardiogram
%K cardiac myoblasts
%K dioc1.
%U http://www.scielo.org.ve/scielo.php?script=sci_abstract&pid=S0798-04692009000200010&lng=en&nrm=iso&tlng=en