%0 Journal Article
%T First isolation of dengue 4 in the state of S£¿o Paulo, Brazil, 2011
%A Rocco
%A Iray Maria
%A Silveira
%A Vivian Regina
%A Maeda
%A Adriana Yurika
%A Silva
%A Sarai Joaquim dos Santos
%A Spenassatto
%A Carine
%A BISORDI
%A Ivani
%A Suzuki
%A Akemi
%J Revista do Instituto de Medicina Tropical de S£¿o Paulo
%D 2012
%I Instituto de Medicina Tropical
%R 10.1590/S0036-46652012000100009
%X we report the first isolation of dengue virus 4 (denv-4) in the state of s£¿o paulo, from two patients - one living in s£¿o jos¨¦ do rio preto and the other one in paulo de faria, both cities located in the northwest region of the state. the virus isolations were accomplished in the clone c6/36 aedes albopictus cell line, followed by indirect immunofluorescence assays, performed with type-specific monoclonal antibodies that showed positive reactions for denv-4. the results were confirmed by nested rt-pcr and real-time rt-pcr assays. the introduction of denv-4 in a country that already has to deal with the transmission of three other serotypes increases the possibility of the occurrence of more severe cases of the disease. the importance of early detection of dengue cases, before the virus spreads and major outbreaks occur, should be emphasized.
%K dengue 4
%K virus isolation
%K rt-pcr.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0036-46652012000100009&lng=en&nrm=iso&tlng=en