%0 Journal Article
%T Manejo de Pl¨¢ntulas de Mango (Mangifera indica L.) Obtenidas in vitro para Estudios de Sanidad Vegetal
%A L¨®pez-Estrada
%A Martha Elena
%A Acosta-Rodr¨ªguez
%A Mar¨ªa Concepci¨®n
%A Otero-Colina
%A Gabriel
%A Michel-Aceves
%A Alejandro Casimiro
%A Noriega-Cant¨²
%A David Heriberto
%J Revista mexicana de fitopatolog¨ªa
%D 2008
%I Scientific Electronic Library Online
%X in vitro mango seedlings free of external and seed-transmitted contaminants were obtained from immature embryos. then, they were transplanted to soil using only sterilized materials. mango embryos 45-60 days old were plated in modified b5 medium; and after seedlings attained 10 cm height, they were transplanted to soil + expanded perlite in specially designed plastic containers. plants were irrigated with nutritive universal solution. during the in vitro stage 10% contamination with penicillium, aspergillus, xanthomonas and cladosporium was observed. contamination in plastic containers gradually reached 15% after five months. cladosporium, alternaria, aspergillus, rhizopus, and fusarium were identified in the substrate, whereas helminthosporium, curvularia, penicillium, and syncephalastrum appeared in the plastic containers.
%K embryo culture
%K contaminants
%K substrate
%K micropropagation
%K disinfection.
%U http://www.scielo.org.mx/scielo.php?script=sci_abstract&pid=S0185-33092008000200012&lng=en&nrm=iso&tlng=en