%0 Journal Article
%T Nanotubo de carbono-chitosan en c¨¦lulas HOS y THP-1
%A Castillo Le¨®n
%A John Jairo
%A Novoa
%A Leidy Viviana
%A Mart¨ªnez Ortega
%A Fernando
%A Escobar Rivero
%A Patricia
%J Revista de la Universidad Industrial de Santander. Salud
%D 2011
%I Universidad Industrial de Santander
%X introduction: carbon nanotubes (cnt) are nanometer-sized structures used in medicine in the treatment of diseases, mainly in drug delivery in therapies against cancer. objectives: to study the internalization of carbon nanotubes modified with chitosan (cnt-ch) in human osteosarcom cells (hos) and human monocytes of acute leukemia (thp1). materials and methods: the cnts solubilized in chitosan 30% were characterized spectroscopically by uv-vis, fluorescence and raman. hos cells and thp-1 were treated with cnt-ch, the internalization was evaluated by giemsa staining with light microscopy, and cytotoxicity was determined using alamar blue assay. results: raman and fluorescence spectra showed the functionalization of the cnt with chitosan. after 24 h the ntc were internalized in the cell lines showing a cytoplasmic location and were not cytotoxic in any of both cell lines types. discussion: the properties of cnt-ch on cells allowing it to be potentially used as drug carrier salud uis 2011; 43(1): 21-26.
%K carbon nanotubes
%K chitosan
%K cell internalization cells
%K macrophages.
%U http://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S0121-08072011000100004&lng=en&nrm=iso&tlng=en