%0 Journal Article
%T Development of DArT markers for a linkage map of flue-cured tobacco
%A XiuPing Lu
%A YiJie Gui
%A BingGuang Xiao
%A YongPing Li
%A ZhiJun Tong
%A Yun Liu
%A XueFei Bai
%A WeiRen Wu
%A Ling Xia
%A Eric Huttner
%A Adrzej Kilian
%A LongJiang Fan
%J Chinese Science Bulletin
%@ 1861-9541
%D 2013
%I 
%R 10.1007/s11434-012-5453-z
%X Tobacco (Nicotiana tabacum) is one of the most economically important nonfood crops, and flue-cured tobacco accounts for approximately 80% of world tobacco production. An extremely narrow genetic diversity in the tobacco pool has led to a low efficiency of PCR-based molecular markers (such as AFLP and SSR). Diversity Arrays Technology (DArT) is a high-throughput hybridisation-based marker system that has been developed in many plants including wheat, which, like tobacco, has a complex genome. In this study, we developed a tobacco DArT chip that included 7680 representative sequence tags based on typical tobacco accessions. The 1076 DArT markers of flue-cured tobacco were identified and most (82.1%) of their polymorphism information contents (PICs) were greater than 0.4. An integrated linkage map that included 851 markers (238 DArT and 613 SSR), which is the highest density map of flue-cured tobacco to date, was constructed. This chip-based DArT system provides an alternative in high-throughput marker genotyping for tobacco.
%K Nicotiana tabacum
%K diversity arrays technology (DArT)
%K linkage map
%K DH population
%K molecular marker
%U http://link.springer.com/article/10.1007/s11434-012-5453-z