%0 Journal Article
%T Optical detection of acetylcholine esterase based on CdTe quantum dots
%A ZhenZhen Chen
%A XiangLing Ren
%A FangQiong Tang
%J Chinese Science Bulletin
%@ 1861-9541
%D 2013
%I 
%R 10.1007/s11434-012-5590-4
%X In this study, we have constructed a simple, sensitive and rapid biosensor for detection of acetylcholine esterase (AChE) based on CdTe quantum dots (QDs). The detection limit of AChE by one-step enzyme reaction based on the thiolglycolic acid (TGA) stabilized QDs (TGA-QDs) was 10 U/L and the linear range was 10每100 and 100每1200 U/L, respectively. The detection limit of AChE by two-step enzyme reaction based on the 3-mercaptopropionic acid (MGA) stabilized QDs (MGA-QDs) was found to be 20 U/L and the linear range was 100每2500 U/L. The experimental conditions of biosensors were optimized, and the detection mechanism was studied. We also detected AChE in serum samples based on TGA-QDs or MGA-QDs. The linear range was 10每140 and 50每1000 U/L, respectively. The excellent performance of this novel biosensor demonstrated that this strategy has prodigious potential to be applied in practice detection of AChE.
%K quantum dots
%K fluorescence
%K detection
%K acetylcholine esterase
%U http://link.springer.com/article/10.1007/s11434-012-5590-4