%0 Journal Article
%T Rapid in vitro regeneration method for Moringa oleifera and performance evaluation of field grown nutritionally enriched tissue cultured plants
%A R. K. Saini
%A N. P. Shetty
%A P. Giridhar
%A G. A. Ravishankar
%J 3 Biotech
%@ 2190-5738
%D 2012
%I Springer
%R 10.1007/s13205-012-0045-9
%X The present investigations were attempted to develop the rapid in vitro micropropagation protocol of Moringa oleifera (Variety-PKM-1) from nodal sections of young, aseptically grown seedlings. Benzyladenine (BA) at 4.44 ¦ÌM was found to be optimal in producing on maximum an average of 9.0 ¡À 1.0 axillary shoots per explant after 15 days of inoculation. A high multiplication rate was established through routine sub culturing of nodal sections explanted from in vitro shoot cultures. In vitro rooting of individual shoot culture was maximum (100%) on medium containing indole-3-acetic acid (IAA) at 2.85 ¦ÌM along with indole-3-butyric acid (IBA) at 4.92 ¦ÌM. Eighty percent of the rooted plants survived after being transplanted in the soil, provided that the potted plantlets were covered with clear polythene bags and kept in a shaded greenhouse for 15 days before exposure to ambient conditions. Fresh leaves of field grown tissue culture plants were analyzed for lutein, ¦Â-carotene, ¦Á-tocopherol, total carotenoids and chlorophyll content. Tissue culture-derived plants were found nutritionally superior over control plants to contain 13.2 and 14.7% higher amount ¦Á-tocopherol and total carotenoids, respectively. The result of present study will be useful for rapid clonal propagation of M. oleifera and production of nutritionally superior plant.
%K Carotenoids
%K Drumstick
%K Horseradish tree
%K Micropropagation
%K Tocopherol
%U http://link.springer.com/article/10.1007/s13205-012-0045-9