%0 Journal Article %T Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide %A Gerard ¨¤lvarez %A Marta Gonz¨˘lez %A Sergio Isabal %A Vanessa Blanc and Rub¨¦n Le¨®n %J AMB Express %D 2013 %I Springer %R 10.1186/2191-0855-3-1 %X In short, we standardised the conditions for using qPCR-PMA in 5 oral bacterial species and proved its usefulness for quantification of live and dead cells in multi-species oral biofilms formed in vitro, after use of an antiseptic. %K Oral biofilm %K Real-time PCR %K Propidium monoazide (PMA) %K Antiseptic %U http://www.amb-express.com/content/3/1/1/abstract