%0 Journal Article
%T An in vitro assay system for antihyperlipidemic agents by evaluating lipoprotein profiles from human intestinal epithelium-like cells
%A Junichiro Takahashi
%A Kikumi Ogihara
%A Yuko Naya
%A Fumiko Kimura
%A Mizuho Itoh
%A Yuka Iwama
%A Yukie Matsumoto
%A Gen Toshima
%A Keishi Hata
%J 3 Biotech
%@ 2190-5738
%D 2013
%I Springer
%R 10.1007/s13205-012-0085-1
%X We developed an in vitro screening system for antihyperlipidemic activity by measuring lipoprotein profiles secreted from human intestinal epithelium-like cells from the colon cancer cell line, Caco-2. Sodium (Na) butyrate at 5 mM differentiated Caco-2 cells into intestinal epithelium-like cells and numerous microvilli on the apical side of cells were observed under transmission electron microscopy. Real-time RT-PCR analysis revealed that Na butyrate stimulated expression levels of intestinal differentiation markers in Caco-2 cells in a dose-dependent manner and 5 mM Na butyrate up-regulated intestinal alkaline phosphatase, sucrase¨Cisomaltase complex, and microsomal triglyceride transfer protein by 8.1-, 1.9-, and 2.1-fold that of non-treated cells, respectively. Lipoprotein secretions from differentiated Caco-2 cells were promoted by lysophosphatidyl choline and Na oleate, which are a stimulator of lipoprotein secretion and a substrate of triglycerides, respectively. We examined the effects of Pluronic L-81, a lipoprotein secretion inhibitor, on lipoprotein profiles of differentiated Caco-2 cells. Pluronic L-81 at 1.0 ¦Ìg/ml inhibited TG contents in lipoprotein fractions from cells by 25.6 % and secretion was completely suppressed by the agent at 10 ¦Ìg/ml.
%K Caco-2 cell differentiation
%K Intestinal cells
%K Lipoprotein profile
%K Triglyceride
%K Antihyperlipidemic agent
%U http://link.springer.com/article/10.1007/s13205-012-0085-1