%0 Journal Article %T Effect of pre-cooking times on enzymes, properties, and melanosis of Pacific white shrimp during refrigerated storage %A Kusaimah Manheem %A Soottawat Benjakul %A Kongkarn Kijroongrojana %A Nandhsha Faithong and Wonnop Visessanguan %J International Aquatic Research %D 2013 %I BioMed Central %R 10.1186/2008-6970-5-1 %X Pre-cooked shrimp have gained interest due to their attractive color and flavor. However, shrimp without sufficient pre-cooking have faced melanosis, particularly during the extended storage. This leads to the rejection by consumers and the market. Therefore, pre-cooking with appropriate time would be a means to tackle such a problem. The objective of this study was to investigate the effect of pre-cooking times on enzyme activities, properties, microstructure, and melanosis of Pacific white shrimp (Litopenaeus vannamei). Pacific white shrimp were subjected to pre-cooking at 80ˇăC for various times (0 to 120 s). The residual activities of polyphenoloxidase (PPO) and protease decreased with increasing pre-cooking times (p < 0.05). Nevertheless, the increasing pre-cooking time resulted in more cooking loss. With cooking time above 60 s, a cooking loss of 9.85% was obtained (p < 0.05). Marked decreases in the relative PPO and protease activities were observed within the first 30 s of pre-cooking, and negligible activities were detected after 120 s. The microstructure study revealed that the muscle fibers of pre-cooked shrimps were less attached with concomitantly higher shrinkage of the sarcomere, compared with those of the raw counterpart. Shrimps pre-cooked with longer time showed a lower development of black spots as evidenced by a lower melanosis score throughout storage of 7 days at 4ˇăC. Therefore, a pre-cooking time of 30 s at 80ˇăC was sufficient to lower PPO and protease activities with the minimized cooking loss and melanosis during refrigerated storage. %K Pre-cooking time %K Pacific white shrimp %K Melanosis %K Polyphenoloxidase %K Protease %K Cooking loss %K Microstructure %U http://www.intaquares.com/content/5/1/1/abstract