%0 Journal Article
%T Micropropagation of Senecio Macrophyllus M. Bieb
%A Alina Trejgell
%A Magdalena Michalska
%A Andrzej Tretyn
%J Acta Biologica Cracoviensia Series Botanica
%@ 1898-0295
%D 2010
%I 
%R 10.2478/v10182-010-0009-5
%X This is the first protocol for in vitro micropropagation of Senecio macrophyllus. Shoot tips and fragments of the cotyledon, hypocotyls and roots were isolated from 10-day-old sterile seedlings. The morphological response was tested on MS medium supplemented with different types of cytokinins: BA (2.2 ¦ÌM, 4.4 ¦ÌM or 13.3 ¦ÌM), KN (4.7 ¦ÌM or 13.9 ¦ÌM) and ZEA (4.6 ¦ÌM or 13,7 ¦ÌM) in combination with 0.54 ¦ÌM NAA or 0.27 ¦ÌM NAA (with 2.2 ¦ÌM BA only), but only shoot tips were capable of shoot organogenesis. Shoot proliferation was highest for explants cultured on MS medium supplemented with 4.4 ¦ÌM BA in combination with 0.54 ¦ÌM NAA. The shoots formed were then multiplied on the same medium. Rooting was achieved on full- and half-strength MS medium without auxin, but shoots cultured on medium BA-supplemented began inducing roots a week later than shoots obtained on media with other types of cytokinins. Well-rooted plantlets were transferred to ex vitro conditions. The survival rate of rooted plants was 100% for plants cultured in a mixture of vermiculite and sand, and 92% for those planted in soil after 4 weeks of acclimatization. In the first year the plants grew intensively under field conditions and were able to develop a leaf rosette. In the second year the plants were able to flower and produce viable seeds.
%K Senecio macrophyllus
%K micropropagation
%K shoot tip
%K benzylaminopurine
%K flowering plantlets
%U http://versita.metapress.com/content/f102372732693262/?p=bc2e3071f7b149109e3fcc5a0e3a6e48&pi=8