%0 Journal Article
%T miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90+ HepG2 cells
%A Lin Fang
%A Hai-Bing Zhang
%A Hua Li
%A Yong Fu
%A Guang-Shun Yang
%J Radiology and Oncology
%@ 1581-3207
%D 2012
%I 
%R 10.2478/v10019-012-0025-z
%X Background. Since the introduction of the theory of tumour stem cells (TSCs), the liver cancer stem cell (LCSC)-like cells have become one of the focuses in the research on liver cancer. Materials and methods. In this study, CD90+ cells were applied as the possible LCSC-like cells, and the miRNA and gene expression were analyzed in the CD90+ HepG2 cells. The pilot study showed miR-548c-5p exerted potential effect on the CD90+ HepG2 cells and was thereafter applied for the further study. CD90+ HepG2 cells were assigned to miR-548c-5p mimic transfection group and control group. MTT assay was performed to detect the proliferation of CD90+ HepG2 cells. The migration and invasion abilities were examined by wound healing assay and transwell migration assay, respectively. A detection of apoptosis was performed by fluorescence microscopy. Results. Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90+ HepG2 cells. Moreover, the miR-548c-5p transfection could down-regulate the expression of ¦Â-catenin, Tg737, bcl-2, bcl-XL, and caspase-3, inhibit the proliferation, migration and invasion and promote the apoptosis of the CD90+ HepG2 cells. Conclusions. Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.
%K liver cancer stem cells
%K miR-548c-5p
%K apoptosis
%K NF-kB
%K ¦Â-catenin
%U http://versita.metapress.com/content/069220081033j748/?p=cd6efcaa04f946e283dc8e644b832829&pi=6