%0 Journal Article
%T Soman and VX: different effect on cellular signalling
%A Jaroslav Pejchal
%A Jan  sterreicher
%A Ji ¨ª Kassa
%A Ale  Tichy
%A Zuzana  inkorov¨¢
%A Lenka Z¨¢rybnick¨¢
%A Kl¨¢ra Kubelkov¨¢
%A Kamil Ku a
%J Journal of Applied Biomedicine
%@ 1214-0287
%D 2012
%I 
%R 10.2478/v10136-011-0018-z
%X The purpose of our study was to examine the early expression of p21 and activated transcription factors ATF-2, CREB, Elk-1, p53 after soman and VX poisoning, to throw light on the pathogenetic mechanism of nerve agent-induced non-specific effects. Male Wistar rats were i.m. poisoned by soman (60 ¦Ìg.kg-1 - 70% LD50) or VX (8 ¦Ìg.kg-1 - 70% LD50). Samples were taken 4, 24, and 72 hours after poisoning, immunohistochemically stained and phospho-ATF-2Thr-69/71, phospho-CREBSer-133, phospho-Elk-1Ser-383, phospho-p53Ser-15, and protein p21 expressions were measured using computer Image analysis in apical and cryptal enterocytes of the colon transversum. After soman poisoning, we observed an increased phospho-CREB in cryptal enterocytes 4, 24, and 72 h after poisoning, while apical enterocytes expressed increased phospho-CREB only 72 h after intoxication. Phospho-Elk-1 significantly dropped 4 and 24 h after soman poisoning in the cryptal compartment. Activation of ATF-2 and p53 and expression of p21 were not changed 4, 24, and 72 h after soman poisoning. VX poisoning did not change any of measured parameters. Soman and VX showed a different effect on cellular signalling. Soman seems to cause additional effects, which are not related to the basic mechanism of nerve agent-induced toxicity and which temporarily suppress promitotic pathways of proliferating cells and persist in cells during the differentiation process.
%K soman
%K VX
%K ATF-2
%K CREB
%K Elk-1
%K p21
%K p53
%K enterocyte
%K image analysis
%U http://versita.metapress.com/content/lp8k8262g8673144/?p=d0b8c0f382f04d4494fc4481bddc3f89&pi=6