%0 Journal Article
%T Using a different growth medium greatly improves distinction of Butyrivibrio fibrisolvens and Pseudobutyrivibrio xylanivorans strains by the cellular fatty acids and aldehydes profiles
%A Ma a Zorec
%A Ma a Vodovnik
%A Romana Marin ek Logar
%J Acta agriculturae Slovenica
%@ 1854-1941
%D 2011
%I University of Ljubljana
%R 10.2478/v10014-011-0009-5
%X A total of 11 ruminal strains currently assigned to Butyrivibrio fibrisolvens and Pseudobutyrivibrio xylanivorans were cultivated in two different media, rumen fluid containing M2 and short-chain fatty acid (SCFA) containing M330, and their cellular fatty acid methyl esters (FAME) and dimethylacetals (DMA) were analyzed using gas chromatography. A comparison of the FAME/DMA compositions revealed that the difference in SCFA contents in the growth medium induced a pronounced quantitative effect on the cellular branched-chain fatty acid and aldehydes proportions only in the P. xylanivorans strains. This study shows that FAME/DMA analysis is a powerful chemotaxonomic tool in the group of phenotypically similar rumen butyrivibria especially when the influence of the growth medium is evaluated.
%K microbiology
%K anaerobic bacteria
%K rumen
%K Butyrivibrio
%K Pseudobutyrivibrio
%K FAME
%K DMA
%U http://versita.metapress.com/content/6878m13573435w63/?p=81981dea7c934002b843e5bd362eb70b&pi=1