%0 Journal Article
%T Production of a Thermostable ¦¡-Amylase and its Assay using Bacillus Licheniformis Isolated from Excavated Land Sites in Ibadan, Nigeria
%A EO Oziengbe
%A AA Onilude
%J Bayero Journal of Pure and Applied Sciences
%D 2012
%I Bayero University
%X Screening for amylolytic properties from obtained isolates was carried out on starch agar plates while production and characterization of ¦Á-amylase was carried out using submerged fermentation. The isolated organism was identified as Bacillus licheniformis. Physiological studies on the isolate showed that temperature of 55oC and pH 7 was optimum for growth of the organism while fructose (1%), peptone (0.5%), pH 7 and temperature of 60oC supported optimum amylase production. Crude characterization of ¦Á-amylase revealed optimum amylolytic activity at pH 7 and a temperature of 70oC. The isolated Bacillus licheniformis produced thermostable ¦Á-amylase with characteristics suitable for use in starch processing other industries.
%U http://www.ajol.info/index.php/bajopas/article/view/80951