%0 Journal Article
%T Prolonged Treatment with Free Fatty Acids has Post Receptor Effect in Hepatic Insulin Resistance: Evidence that Fatty Acids, Oleate and Palmitate have Insignificant Effect on the Insulin Receptor Beta In Vivo and Ex Vivo Primary Hepatocytes
%A Rafik Ragheb
%A Amina M. Medhat
%A Gamila M.L. Shanab
%A Dina M. Seoudi and Fantus I.G.
%J Biochemistry Insights
%D 2012
%I 
%X In the current study, we used immunoprecipitation and immunoblotting to examine the levels and phosphorylation status of the insulin receptor-beta subunit (IR-¦Â), as well as the down stream target in PI3K pathway, total PKB/Akt as well as their phosphorylated forms. The assessment of FFAs treatment showed no direct and significant effect on the PI3K stimulation, specifically the IR-¦Â in primary hepatic control cells treated with insulin. Cells treated with either oleate or palmitate (360 ¦ÌM) showed no statistically significant values following insulin stimulation (P > 0.05). To further investigate the effect of both FFAs and high insulin (1 ¦Ìg), we examined the effects of oleate and palmitate at 360 ¦ÌM concentration on IR-¦Â as well as PKB. There was no significant difference in the total protein levels and their phosphorylated forms in cells treated with or without oleate or plamitate. Interestingly, IR-¦Â tyrosine phosphorylation showed a similar insignificant effect in vivo and ex vivo hepatic cells treated with oleate or palmitate in comparison to their controls in the fructose fed hamsters.
%U http://www.la-press.com/prolonged-treatment-with-free-fatty-acids-has-post-receptor-effect-in--article-a1497