%0 Journal Article
%T Rapid Isocratic Liquid Chromatographic Separation and Quantification of Tryptophan and Six kynurenine Metabolites in Biological Samples with Ultraviolet and Fluorimetric Detection
%A Abdulla A-B Badawy and Christopher J Morgan
%J International Journal of Tryptophan Research
%D 2012
%I 
%R 10.4137/IJTR.S6225
%X A simple, rapid isocratic liquid chromatographic procedure with ultraviolet and fluorimetric detection is described for the separation and quantification of L-tryptophan (Trp) and six of its kynurenine metabolites (kynurenine, 3-hydroxykynurenine, and 3-hydroxyanthranilic, kynurenic, xanthurenic and anthranilic acids). Using the Perkin Elmer LC 200 system, a reverse phase Synergi 4 ¦Ì fusion-RP80 A column (250 ¡Á 4.6 mm) (Phenomenex), and a mobile phase of 10 mM sodium dihydrogen phosphate: methanol (73:27, by vol) at pH 2.8 and a flow rate of 1.0¨C1.2 ml/min at 37 ¡ãC, a run took ~13 min. The run took <7 min at 40 ¡ãC and a 1.4 ml/min flow rate. Limits of detection of all 7 analytes were 5¨C72 nM and their recoveries from human plasma and rat serum and liver varied between 62% and 111%. This simple method is suitable for high throughput work and can be further developed to include quinolinic acid and other Trp metabolites.
%U http://www.la-press.com/rapid-isocratic-liquid-chromatographic-separation-and-quantification-o-article-a2391