%0 Journal Article
%T Alpha-Tocopherol Alters Transcription Activities that Modulates Tumor Necrosis Factor Alpha (TNF-a) Induced Inflammatory Response in Bovine Cells
%A Cong-Jun Li
%A Robert W. Li
%A Stanislaw Kahl and Theodore H. Elsasser
%J Gene Regulation and Systems Biology
%D 2012
%I 
%R 10.4137/GRSB.S8303
%X To further investigate the potential role of ¦Á-tocopherol in maintaining immuno-homeostasis in bovine cells (Madin-Darby bovine kidney epithelial cell line), we undertook in vitro experiments using recombinant TNF-¦Á as an immuno-stimulant to simulate inflammation response in cells with or without ¦Á-tocopherol pre-treatment. Using microarray global-profiling and IPA (Ingenuity Pathways Analysis, Ingenuity  Systems, http://www.ingenuity.com) data analysis on TNF-¦Á-induced gene perturbation in those cells, we focused on determining whether ¦Á-tocopherol treatment of normal bovine cells in a standard cell culture condition can modify cell's immune response induced by TNF-¦Á challenge. When three datasets were filtered and compared using IPA, there were a total of 1750 genes in all three datasets for comparison, 97 genes were common in all three sets; 615 genes were common in at least two datasets; there were 261 genes unique in TNF-¦Á challenge, 399 genes were unique in ¦Á-tocopherol treatment, and 378 genes were unique in the ¦Á-tocopherol plus TNF-¦Á treatment. TNF-¦Á challenge induced significant change in gene expression. Many of those genes induced by TNF-¦Á are related to the cells immune and inflammatory responses. The results of IPA data analysis showed that ¦Á-tocopherol-pre-treatment of cells modulated cell's response to TNF-¦Á challenge. In most of the canonical pathways, ¦Á-tocopherol pretreatment showed the antagonistic effect against the TNF-¦Á-induced pro-inflammatory responses. We concluded that ¦Á-tocopherol pre-treatment has a significant antagonistic effect that modulates the cell's response to the TNF-¦Á challenge by altering the gene expression activities of some important signaling molecules.
%U http://www.la-press.com/alpha-tocopherol-alters-transcription-activities-that-modulates-tumor--article-a2942