%0 Journal Article
%T White Blood Cell Differentials Enrich Whole Blood Expression Data in the Context of Acute Cardiac Allograft Rejection
%A Casey P. Shannon
%A Zsuzsanna Hollander
%A Janet Wilson-McManus
%A Robert Balshaw
%A Raymond T. Ng
%A Robert McMaster
%A Bruce M. McManus
%A Paul A. Keown
%A and Scott J. Tebbutt
%J Bioinformatics and Biology Insights
%D 2012
%I 
%R 10.4137/BBI.S9197
%X Acute cardiac allograft rejection is a serious complication of heart transplantation. Investigating molecular processes in whole blood via microarrays is a promising avenue of research in transplantation, particularly due to the non-invasive nature of blood sampling. However, whole blood is a complex tissue and the consequent heterogeneity in composition amongst samples is ignored in traditional microarray analysis. This complicates the biological interpretation of microarray data. Here we have applied a statistical deconvolution approach, cell-specific significance analysis of microarrays (csSAM), to whole blood samples from subjects either undergoing acute heart allograft rejection (AR) or not (NR). We identified eight differentially expressed probe-sets significantly correlated to monocytes (mapping to 6 genes, all down-regulated in ARs versus NRs) at a false discovery rate (FDR) ¡Ü 15%. None of the genes identified are present in a biomarker panel of acute heart rejection previously published by our group and discovered in the same data.
%U http://www.la-press.com/white-blood-cell-differentials-enrich-whole-blood-expression-data-in-t-article-a3126