%0 Journal Article
%T Poly(ADP-ribose) Preparation Using Anion-Exchange Column Chromatography
%A Takashi Shimokawa
%A Hideki Ogino
%A Daisuke Maeda
%A Hitoshi Nakagama
%A Takashi Sugimura and Mitsuko Masutani
%J Organic Chemistry Insights
%D 2012
%I 
%X Poly(ADP-ribose) polymerase (PARP) polyADP-ribosylates proteins involved in various physiological processes. Accumulated evidence suggests not only protein-conjugated poly(ADP-ribose) but also protein-free poly(ADP-ribose) function in various physiological processes. There are increasing occasions that require protein-free poly(ADP-ribose) to study the function and dynamics of poly(ADP-ribose) in cells. However, the availability of poly(ADP-ribose) is still limited because a chemical synthesis method has not been established. Here, we describe an improved method for the preparation of protein-free poly(ADP-ribose), synthesized enzymatically by using a recombinant PARP-1 expression system and purified with an anion-exchange column chromatography. This method will be useful for biochemical and biological investigation of poly(ADP-ribose) functions and dynamics.
%U http://www.la-press.com/polyadp-ribose-preparation-using-anion-exchange-column-chromatography-article-a1409