%0 Journal Article
%T Stereoselective Reduction of ¦Á-Keto Ester and ¦Á-Keto Amide with Marine Actinomycetes, Salinispora Strains, as Novel Biocatalysts
%A Kohji Ishihara
%A Hirokazu Nagai
%A Kazunari Takahashi
%A Mariko Nishiyama and Nobuyoshi Nakajima
%J Biochemistry Insights
%D 2012
%I 
%R 10.4137/BCI.S7877
%X To clarify the potential ability of marine actinomycetes as biocatalysts, the stereoselective reduction of ¦Á-keto esters and ¦Á-keto amide using Salinispora arenicola and Salinispora tropica was tested. The reduction of ethyl pyruvate and ethyl 2-oxobutanoate by S. tropica gave corresponding alcohol with high conversion ratio and in high e.e. (96% e.e. (S) and 99% e.e. (S), respectively). In the presence of l-glutamate as an additive, the reduction of ethyl pyruvate by S. tropica afforded the corresponding (S)-ethyl lactate with >99% e.e. Furthermore, 2-chlorobenzoylformamide was reduced by S. tropica to the corresponding (R)-2-chloromandelamide with high conversion ratio and excellent enantioselectivity (>99% e.e.). Thus, it was found that marine actinomycetes, Salinispora strains, had high ability for the stereoselective reduction of carbonyl compounds as useful biocatalysts.
%U http://www.la-press.com/stereoselective-reduction-of--keto-ester-and--keto-amide-with-marine-a-article-a2812