%0 Journal Article
%T Optimized Protocol for Protein Extraction from the Breast Tissue that is Compatible with Two-Dimensional Gel Electrophoresis
%A Olena Zakharchenko
%A Christina Greenwood
%A Louise Alldridge and Serhiy Souchelnytskyi
%J Breast Cancer: Basic and Clinical Research
%D 2012
%I 
%R 10.4137/BCBCR.S6263
%X Proteomics is a highly informative approach to analyze cancer-associated transformation in tissues. The main challenge to use a tissue for proteomics studies is the small sample size and difficulties to extract and preserve proteins. The choice of a buffer compatible with proteomics applications is also a challenge. Here we describe a protocol optimized for the most efficient extraction of proteins from the human breast tissue in a buffer compatible with two-dimensional gel electrophoresis (2D-GE). This protocol is based on mechanically assisted disintegration of tissues directly in the 2D-GE buffer. Our method is simple, robust and easy to apply in clinical practice. We demonstrate high quality of separation of proteins prepared according to the reported here protocol.
%U http://www.la-press.com/optimized-protocol-for-protein-extraction-from-the-breast-tissue-that--article-a2527