%0 Journal Article
%T Characterization of Non-Conserved HLA-A*0201 Binding T cell Epitopes of JC Virus T Antigen
%A Jongming Li
%A John L. Wagner and Bijoyesh Mookerjee
%J Virology: Research and Treatment
%D 2012
%I 
%X JC virus-specific CD8+ cytotoxic T lymphocytes are associated with a favorable outcome in patients with progressive multifocal leukoencephalopathy. However, very few JC virus T cell epitopes restricted to MHC class I have been defined. Of the two HLA-A*0201-restricted JCV epitopes, VP1p36 and VP1p100, studies have shown that they are conserved T cell epitopes of polyomaviruses. The cross-recognition associated to these epitopes has complicated the efforts of understanding the dynamics of immune response to JC virus. Based on the previously identified HLA-A*0201 binding T cell epitope of Simian virus 40 T antigen P281每289 (KCDDVLLLL) and BK virus T antigen P558每566 (SLQNSEFLL), T cell epitopes of JC Virus T antigen P282每290 (KCEDVFLLM) and P557每565 (SLSCSEYLL) were identified. In this report, we demonstrated that JC Virus P282每290 P557每565 were able to stimulate T cell responses in healthy donors＊ PBMCs and CD8+ cytotoxic T lymphocytes raised with both peptides could recognize and lyse their targets. Most importantly, there were no T cell cross-recognitions between JC Virus, BK Virus and SV40 virus. Therefore, JCV T-ag epitopes P282每290 and P557每565 could be better antigen epitopes compared to VP1p36 and VP1p100 to study the dynamics of cellular immune response to JCV in PML patients. In addition, as a HLA-A*0201 binding T cell epitope, both peptides could be a valuable component of immunotherapies aiming at increasing the cellular immune response against JCV for the treatment of progressive multifocal leukoencephalopathy.
%U http://www.la-press.com/characterization-of-non-conserved-hla-a0201-binding-t-cell-epitop-article-a1000