%0 Journal Article
%T A Rapid Stability-Indicating RP-HPLC Method for the Determination of Betaxolol Hydrochloride in Pharmaceutical Tablets
%A Sylvain Auvity
%A Fouad Chiadmi
%A Salvatore Cisternino
%A Jean-Eudes Fontan and Jo l Schlatter
%J Analytical Chemistry Insights
%D 2012
%I 
%R 10.4137/ACI.S11256
%X A stability-indicating reversed-phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of betaxolol hydrochloride, a drug used in the treatment of hypertension and glaucoma. The desired chromatographic separation was achieved on a Nucleosil C18, 4 ¦Ìm (150 ¡Á 4.6 mm) column, using isocratic elution at a 220 nm detector wavelength. The optimized mobile phase consisted of a 0.02 M potassium dihydrogen phosphate: methanol (40:60, v/v, pH 3.0 adjusted with o-phosphoric acid) as solvent. The flow rate was 1.6 mL/min and the retention time of betaxolol hydrochloride was 1.72 min. The linearity for betaxolol hydrochloride was in the range of 25 to 200 ¦Ìg/mL. Recovery for betaxolol hydrochloride was calculated as 100.01%¨C101.35%. The stability-indicating capability was established by forced degradation experiments and the separation of unknown degradation products. The developed RP-HPLC method was validated according to the International Conference on Harmonization (ICH) guidelines. This validated method was applied for the estimation of betaxolol hydrochloride in commercially available tablets.
%U http://www.la-press.com/a-rapid-stability-indicating-rp-hplc-method-for-the-determination-of-b-article-a3587