%0 Journal Article
%T Alterations with age in peripheral blood lymphocyte subpopulations and cytokine synthesis in beagles
%A Fujiwara M
%A Yonezawa T
%A Arai T
%A Yamamoto I
%A Ohtsuka H
%J Veterinary Medicine: Research and Reports
%D 2012
%I Dove Medical Press
%R http://dx.doi.org/10.2147/VMRR.S32590
%X lterations with age in peripheral blood lymphocyte subpopulations and cytokine synthesis in beagles Original Research (1111) Total Article Views Authors: Fujiwara M, Yonezawa T, Arai T, Yamamoto I, Ohtsuka H Published Date August 2012 Volume 2012:3 Pages 79 - 84 DOI: http://dx.doi.org/10.2147/VMRR.S32590 Received: 30 May 2012 Accepted: 14 July 2012 Published: 27 August 2012 Megumi Fujiwara,1,2 Tomohiro Yonezawa,3 Toshiro Arai,1 Ichiro Yamamoto,1 Hiromichi Ohtsuka2 1Laboratory of Veterinary Biochemistry, Nippon Veterinary and Life Science University, Tokyo, Japan; 2Laboratory of Large Animal Internal Medicine, 3Laboratory of Veterinary Physiology, Kitasato University, Towada, Japan Purpose: The immune system is considered to be affected by aging, which is linked to various immune pathogeneses. The purpose of this study was to determine age-associated changes in immune function of healthy dogs (beagles), specifically those of naive and memory T lymphocytes, based on cytokine synthesis. Patients and methods: Blood samples were obtained from 44 healthy beagles that were divided into three age-groups: young (<4 years), middle-aged (4¨C8 years), and older dogs (>8 years). Subpopulations of T lymphocytes were determined by flow cytometry. Transcriptional (mRNA) levels of cytokines were determined for primary-cultured leukocytes using quantitative real-time polymerase chain reaction. Results: There were negative correlations between dogs¡¯ages and the number of peripheral blood mononuclear cells, T cells, and B cells. In particular, the number of naive CD4+ CD45RA+ T cells and CD8+ CD45RA+ T cells significantly decreased with age. The mRNA levels for interleukin (IL)-2, IL-2R¦Á, and interferon-gamma were significantly higher in young or middle-aged dogs (P < 0.05), whereas IL-4 mRNA expression was not significantly different over the different age-groups. IL-2R¦Ã mRNA expression tended to decrease with age. Conclusion: Decreases of naive CD4+ and naive CD8+ T cells may be related to age-related immunosenescence in dogs. With regard to cytokine production, leukocyte IL-4 and IL-10 mRNA levels did not change with age, whereas IL-2, IL-2R¦Á, and IL-2R¦Ã mRNA levels decreased with age. These altered cytokine mRNA expression patterns may contribute to decreased naive T-cell function(s) with aging.
%K aging
%K leukocyte subpopulation
%K cytokine
%K dog
%K naive T cell
%K IL-2R
%U https://www.dovepress.com/alterations-with-age-in-peripheral-blood-lymphocyte-subpopulations-and-peer-reviewed-article-VMRR