%0 Journal Article
%T Molecular Characterization of Seven Different Species of Aspergillus through Random Amplified Polymorphic DNA (RAPD) and Enzyme Analysis
%J Journal of Microbiology Research
%@ 2166-5931
%D 2012
%I 
%R 10.5923/j.microbiology.20120203.01
%X The aim of this study was to characterize seven species of Aspergillus at molecular level, using random amplification of polymorphic DNA (RAPD). RAPD- PCR conditions were optimized for two primers of series B, GL Decamer B-09 and GL Decamer B-10 out of 10 total primers. RAPD results were evaluated by a statistical software Minitab and a phylogenetic tree was prepared. GL Decamer B-09 showed 38 bands and GL Decamer B-10 gave 46 bands, showing 50% and 57% similarity respectively, among species. Biochemical characterization was done by screening of zones production with particular enzyme activity of each specie resulted in particular substrate degradation.
%K Aspergillus
%K RAPD
%K Minitab
%K Pectinase activity
%K Phylogeny
%U http://article.sapub.org/10.5923.j.microbiology.20120203.01.html